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pSAH61 - [ASELp | wrmScarlet | tbb-2 UTR]

Plasmid

#200335

Purpose

mScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Insert

[ASELp | wrmScarlet | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Promoter

WBGene00001534, gcy-7

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBN262

Plasmid

#86720

Purpose

Heat shock-induced FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Phsp-16.41::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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TU#711

Plasmid

#15279

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czgfp

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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TU#708

Plasmid

#15277

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czgfp

Use

Tags

Expression

Worm

Mutation

leucine zipper fused to c-terminal half of gfp

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pJH4330

Plasmid

#191700

Purpose

Punc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Punc-17

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-SNAP-Smo

Plasmid

#31617

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

SNAP

Expression

Mammalian and Worm

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

Plasmid

#107137

Purpose

Dominant negative PKA cGAL effector for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

kin-2a(G310D)::SL2::GFP

Use

Tags

Expression

Worm

Mutation

Promoter

15xUAS-delta-pes-10(basal promoter)

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Plasmid

#159846

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1659

Plasmid

#154314

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::AID*::3xFLAG for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4329

Plasmid

#191679

Purpose

Prgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GtACR2

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pregf-1

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMB67

Plasmid

#47947

Purpose

Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

hsp-16.48

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Plasmid

#159874

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

Plasmid

#182355

Purpose

mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Plasmid

#159866

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDD372

Plasmid

#91824

Purpose

Codon-optimized GFP donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

GFP-C1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGC94

Plasmid

#19610

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.2::FLP::let-858(3’)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCM3.19

Plasmid

#19401

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Sep 23. ():.

Insert

tbb-2 ORF+3' in pDONR201 (tbb-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pMB63

Plasmid

#47945

Purpose

Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pdestDD03

Plasmid

#26414

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2 cassette

Use

Tags

Expression

Bacterial and Worm

Mutation

Promoter

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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