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pEY77

Plasmid

#191072

Purpose

dop-3 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dop-3 (dop-3 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY72

Plasmid

#191069

Purpose

cho-1(3.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.7k) (cho-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY81

Plasmid

#191076

Purpose

flp-32 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-32 (flp-32 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY75

Plasmid

#191071

Purpose

cng-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cng-3 (cng-3 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY67

Plasmid

#191064

Purpose

ceh-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-1 (ceh-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY69

Plasmid

#191066

Purpose

ceh-24 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-24 (ceh-24 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pIN3_enpp-1_mScarlet

Plasmid

#182934

Purpose

Expresses enpp-1 (C27A7.1) under its native promoter (male reproductive tract and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

genomic enpp-1 fused to mScarlet

Use

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Mutation

Promoter

enpp-1

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2

Plasmid

#178969

Purpose

Expresses miRFP2 in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmiRFP2

Use

Tags

no

Expression

Worm

Mutation

NO

Promoter

tag-168 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS640

Plasmid

#188892

Purpose

ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9, Cbr-unc-119

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS1272

Plasmid

#188891

Purpose

Ric-4 sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pIN2_mcm-3_mScarlet

Plasmid

#182933

Purpose

Expresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

genomic mcm-3 fused to mScarlet

Use

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Mutation

Promoter

mcm-3

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pIN1_GGGSx3_mScarlet

Plasmid

#182936

Purpose

C-terminal mScarlet with flexible linker (GGGGS)x3

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

Plasmid

#159842

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI Pmlc-2_GFP target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-Peft-pHCountdown-unc54

Plasmid

#182304

Purpose

C. elegans plasmid encoding pH-Countdown with three introns

Depositor

Andrew York

Article

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH countdown with 3 introns, with a Peft3 promoter and unc54 terminator

Use

Tags

Expression

Worm

Mutation

Promoter

Peft-3

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pdestMB14_grh-1p-grh-1-gfp

Plasmid

#177761

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pdestMB14_hsp-16.2p-grh-1-gfp

Plasmid

#177762

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZ157

Plasmid

#163639

Purpose

pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

pcn-1 (pcn-1 Nematode)

Use

CRISPR

Tags

GFP

Expression

Worm

Mutation

Promoter

pcn-1

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWZ186

Plasmid

#163641

Purpose

rps-27>DHB::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB:2xmKate2::3xHA

Use

CRISPR

Tags

2xmKate2

Expression

Worm

Mutation

Promoter

rps-27

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Use

Tags

2XGFP

Expression

Worm

Mutation

Promoter

nlf-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2198

Plasmid

#179773

Purpose

Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::GFP (nlf-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2437

Plasmid

#179780

Purpose

Prgef-1 NLF-1 unc-54 3' UTR C.elegans pan-neural expression of NLF

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1 (nlf-1 Nematode)

Use

Tags

no

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH3137

Plasmid

#179617

Purpose

Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

GCaMP6

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Punc-4

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3453

Plasmid

#179577

Purpose

Pglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Plgc-55B

Available since

March 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJH3410

Plasmid

#179575

Purpose

Punc-129(DB)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN -specific expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Punc-129

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH3458

Plasmid

#179573

Purpose

Pttr-39::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans D-MN -specific expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

BFP

Expression

Worm

Mutation

Promoter

Pttr-39

Available since

March 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJH2890

Plasmid

#179561

Purpose

Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Plgc-55B

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH2108

Plasmid

#179252

Purpose

Punc-4::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Punc-4

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH2844

Plasmid

#179541

Purpose

Punc-25::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans D-MN and other neurals expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Punc-25

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH2842

Plasmid

#179540

Purpose

Pacr-5::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN and other neurals expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pacr-5

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge