Addgene

New search

Keep filters

1,735 results

Showing: 1441 - 1470 of 1735 results

Results per page:

Sort results by:

pJJF495_dpy-10_CDS_sg6

Plasmid

#164268

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6 promoter from W05B2.8

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF449_dpy-10_CDS_sg1

Plasmid

#163866

Purpose

sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the coding sequence of dpy-10

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF328_sqt-3_3′UTR_sg2

Plasmid

#163867

Purpose

sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting the 3'UTR of sqt-3

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF152_Phsp_Cas9

Plasmid

#163862

Purpose

heat shock-induced expression of Cas9 in C. elegans

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

Cas9, C. elegans codon optimized with intron and SV40_NLS

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Phsp-16.48

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

R07E5.16 (U6)

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Plasmid

#159852

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Plasmid

#159844

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159865

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2365 - Intron GG1 - 250bp no PATC

Plasmid

#159883

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 250bp no PATC

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2345 - Intron GG2 - 250bp no PATC

Plasmid

#159884

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 250bp no PATC

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2366 - intron GG3 - 250bp no PATC

Plasmid

#159885

Purpose

Golden Gate compatible intron with no PATCs for negative controls

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp no PATC

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)

Plasmid

#159888

Purpose

Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

[4-1] - Promoter - Pmlc-1 (w ATG)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)

Plasmid

#159889

Purpose

Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

[4-1] - Promoter - Pmlc-2(no_start)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Plasmid

#159794

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159825

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159829

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM271 - MCS mosTI NeoR target

Plasmid

#159830

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI NeoR target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM322 - array co-injection fragment mosTI neoR

Plasmid

#159832

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI neoR

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159833

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

Plasmid

#159836

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI Pmlc-2_GFP

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA847 - mec-4p::hrp-1mScarlet

Plasmid

#139201

Purpose

Expresses mScarlet tagged hrp-1 in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA844 - hrp-1p::hrp-1mScarlet

Plasmid

#139204

Purpose

Expresses mScarlet tagged hrp-1

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge