7,933 results

1. pCEV-G3-Km

   Plasmid

   #46817

   Purpose

   TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation

   Depositor

   Lars Nielsen, Claudia Vickers

   Article

   Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

   Type

   Empty backbone

   Use

   Tags

   FLAG and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 and GAL10

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKT0128

   Plasmid

   #8729

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yEGFP
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDS275

   Plasmid

   #34972

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T406A, T407A, I532A

   Promoter

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-5FLAG-bleMX6

   Plasmid

   #19344

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   bleMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL358

   Plasmid

   #169147

   Purpose

   Encodes OptoQ-AMP1

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-hygMV

   Plasmid

   #73891

   Purpose

   fission yeast drug selection marker

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Insert

   hygMV
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pombe TEF

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRG646_lexO-HO_LexA-TF_LEU2MX

   Plasmid

   #154815

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIS3

   Plasmid

   #92173

   Purpose

   Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

   Depositor

   Judith Berman

   Article

   Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

   Insert

   NAT1
   

   Use

   Genbank: u03440.1

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXP420

    Plasmid

    #26844

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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