8,060 results

1. pROS14

   Plasmid

   #107928

   Purpose

   LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS315Nab3

   Plasmid

   #110532

   Purpose

   Expresses yeast NAB3 using the endogenous promoter

   Depositor

   Daniel Reines

   Article

   Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

   Insert

   NAB3 (NAB3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIV489

   Plasmid

   #177697

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. YIplac211-iGFP-PIK1

   Plasmid

   #105260

   Purpose

   Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Pik1 (PIK1 Budding Yeast)
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. POM42-mTFP1

   Plasmid

   #69852

   Purpose

   N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mTFP1 minus start and stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. HCP5

   Plasmid

   #166107

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

   Depositor

   Matthias Heinemann

   Insert

   Cyr1CT-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Use

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB2227

   Plasmid

   #67536

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH734-2µ-RLuc/stopCFLuc

   Plasmid

   #40609

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase (nonsense mutant)
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIM572

    Plasmid

    #74640

    Purpose

    alias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB2190

    Plasmid

    #67535

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS-Dhh1-PA (405)

    Plasmid

    #121417

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GTT-g

    Plasmid

    #81109

    Purpose

    Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK59-ATG18-GFP

    Plasmid

    #42529

    Depositor

    James Hurley

    Article

    Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

    Insert

    Atg18 (ATG18 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    none

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGAN101

    Plasmid

    #131123

    Purpose

    Plasmid for overexpression of prion domains to induce prions

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    mKate2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GTH-ir

    Plasmid

    #81107

    Purpose

    Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    tdiRFP
    

    Use

    Cre/Lox

    Tags

    tdiRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB2903(XI-2 MarkerFree)

    Plasmid

    #73275

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSNAPf-AP

    Plasmid

    #61830

    Purpose

    Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    SNAP-AP
    

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pTH726-CEN-RLuc/minCFLuc

    Plasmid

    #38210

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge