8,173 results
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pJPS2957
Plasmid#135591PurposeSNR6 has GAGAUUUAUUUC at position 96–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
ExpressionYeastMutationU6Δ12 has GAGAUUUAUUUC at position 96–107 deletedPromoterNativeAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG569
Plasmid#158588PurposetNCS-ePTS1 Cen6/ARS4DepositorInserttNCS-ePTS1
ExpressionYeastAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPL1B-Cl
Plasmid#160430PurposeExpresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoterDepositorInsertRPL1B
TagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to vary nucleoti…PromoterS. cerevisiae GAL1Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD217
Plasmid#160939PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mTagBFP2-HA Tag
TagsHA tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD118
Plasmid#160938PurposeExcess Capsid Protein in vivo mRNA displayDepositorInsertMCP-mCherry-FLAG tag
TagsMYC tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD115
Plasmid#160937PurposeExcess Capsid Protein for in vivo mRNA displayDepositorInsertMCP-mCherry-MYC tag
TagsMYC tagExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD163
Plasmid#160935PurposeIn vivo mRNA display construct for mCherryDepositorInsertMCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1160c2
Plasmid#159724PurposeExpress MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MPGIL-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-VTS-US
Plasmid#160425PurposeExpression of c-luciferase from sp. Vargula tsujii (USA)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-MSVU-BZ
Plasmid#160426PurposeExpression of c-luciferase from sp. Maristella sp. SVU (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1059
Plasmid#158595PurposetNCS-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)DepositorInsertstNCS-ePTS1
Ec_CYP80B1
Ps_6OMT
Ps_4'OMT2
Ps_CNMT
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1060
Plasmid#158596PurposetNCS-dead-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)DepositorInsertstNCS-dead_ePTS1
Ec_CYP80B1
Ps_6OMT
Ps_4'OMT2
Ps_CNMT
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1067
Plasmid#158597PurposetNCS-Venus (CEN6/ARS4)DepositorInserttNCS-Venus-ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJB207
Plasmid#158599PurposeUbiY-Venus-ePTS1DepositorInsertUbiY-Venus-ePTS1
TagsUbi-Y and ePTS1ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG1066
Plasmid#158590PurposetNCS-Venus-ePTS1 CEN6/ARS4DepositorInserttNCS-Venus-ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAS1643
Plasmid#158591PurposetNCS-VioE CEN6/ARS4DepositorInserttNCS-VioE-ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAS1644
Plasmid#158592PurposetNCS-VioE-dead_ePTS1 CEN6/ARS4DepositorInserttNCS-VioE-dead_ePTS1
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP
Plasmid#154368PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
ExpressionYeastAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE45
Plasmid#139424PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.DepositorInsertmTurquoise1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE44
Plasmid#139423PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.DepositorInsertmTFP1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE43
Plasmid#139422PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.DepositorInsertmMaroon1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1674
Plasmid#159726PurposeExpress MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MPTLV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1479a3
Plasmid#159725PurposeExpress MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MYITV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSJ182
Plasmid#159723PurposeExpress Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCgDepositorInsertGID4 (VID24 Budding Yeast)
UseYeast two-hybrid assayTags3xflag and Gal4ADExpressionYeastMutationremove stop codonPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSJ392
Plasmid#159722PurposeExpress Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCgDepositorInsertGID10 (GID10 Budding Yeast)
UseYeast two-hybrid assayTags3xflag and Gal4ADExpressionYeastMutationremove stop codonPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
TagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1B-mCherry-StreptagII
Plasmid#158744PurposeExpresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-B (KCNK4 Human)
TagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-290 and N-linked glycosylation sites r…PromoterAOX1Available SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 A
Plasmid#129485PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 A (GLO3 Budding Yeast)
ExpressionYeastMutationMutation of serines 389 and 398 to alanineAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 D
Plasmid#129486PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 D (GLO3 Budding Yeast)
ExpressionYeastMutationMutation of serines 389 and 398 to AspAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Met25 Glo3 ΔGRM-ΔC
Plasmid#129487PurposeExpression in S. cerevisiaeDepositorInsertGlo3 ΔGRM ΔC (GLO3 Budding Yeast)
ExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K
Plasmid#112652PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K
ExpressionYeastMutationR59K (GAP-dead)PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K ΔC
Plasmid#112653PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔC
ExpressionYeastMutationR59K, Δ431-493 (deletion of C-terminal amphipathi…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K ΔGRM-ΔC
Plasmid#112654PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔGRM ΔC
ExpressionYeastMutationR59K, Δ376-493 (deletion of Glo3 motif & C-te…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K Δ2x+ve
Plasmid#112655PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K Δ2x+ve
ExpressionYeastMutationR59K, mutations (R223E, K224E, K225E, K233E, K234…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 A
Plasmid#112656PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398A
ExpressionYeastMutationR59K, mutations (S389A, S398A) in Glo3 motif, non…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 D
Plasmid#112657PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398D
ExpressionYeastMutationR59K, mutations (S389D, S398D) in Glo3 motif, pho…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 ΔGRM-ΔC
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
TagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 A
Plasmid#112660PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 S389,398A
TagssfGFPExpressionYeastMutationmutations (S389A, S398A) in Glo3 motif, non-phosp…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
TagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
TagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K
Plasmid#112647PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K
ExpressionYeastMutationR54KPromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K L246D
Plasmid#112648PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K L246D
ExpressionYeastMutationR54K & L246D (in ALPS motif)PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K AxxA
Plasmid#112649PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K AxxA
ExpressionYeastMutationR54K & Alanine substitution of C-terminal δL …PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 Δ3xF AxxA
Plasmid#112650PurposeExpression S.cerevisiaeDepositorInsertGcs1 Δ3xF AxxA
ExpressionYeastMutationR54K, W304A, W307A, W310A, W349A, F353APromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT140_GalL_cCDA1-VRERBE3
Plasmid#145103PurposeExpressing base editor cCDA1-VRERBE3 in yeast cellsDepositorInsertcCDA1-VRERBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT46_GalL_cCDA1-BE3
Plasmid#145039PurposeExpresses cCDA1-BE3 in yeast cellsDepositorInsertcCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME5000
Plasmid#129661PurposeEYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only
