8,060 results

1. pJW1673

   Plasmid

   #112047

   Purpose

   CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   SCT1
   

   Use

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVG48

   Plasmid

   #164706

   Purpose

   rtTA under the control of PGK1 promoter

   Depositor

   Sahand Jamal Rahi

   Article

   Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

   Insert

   PGK1pr - rtTA - CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVP64

   Plasmid

   #22287

   Depositor

   Louise Prakash

   Article

   Pages et al Genes Dev. 2009 Jun 15. 23(12):1438-49.

   Insert

   REV1 (REV1 Budding Yeast)
   

   Use

   Tags

   4xHA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 22, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pGRE GFP

   Plasmid

   #1196

   Depositor

   Susan Lindquist

   Article

   Patino et al Science 1996 Aug 2;273(5275):622-6.

   Insert

   GFP w/ stop codon
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GFP w/ stop codon

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pNB330

   Plasmid

   #60147

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   NLuc
   

   Use

   Tags

   Nuclear localization signal (NLS)

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-bleMX6-P81nmt1-3FLAG

   Plasmid

   #19353

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   bleMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB813

   Plasmid

   #60161

   Purpose

   Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   Promoter

   SIC1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p416TEF-SEC16

   Plasmid

   #102676

   Purpose

   The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

   Depositor

   Jens Nielsen

   Article

   Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

   Insert

   SEC16 (SEC16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426GPD-ccdB-EYFP

   Plasmid

   #14228

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDS191

    Plasmid

    #34978

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pI3-MTU-DO-HPH

    Plasmid

    #160207

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pI3-MTU-DO-LEU

    Plasmid

    #160176

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pI4-MTU-DO-LEU

    Plasmid

    #160029

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pI3-MTU-DO-HIS

    Plasmid

    #160021

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pI3-MTU-DO-URA

    Plasmid

    #160020

    Purpose

    Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pML104-kanR1136

    Plasmid

    #162040

    Purpose

    Expresses Cas9 and contains kanR1136 guide which targets the kanR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    kanR1136 guide
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046207

    Plasmid

    #145559

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. PP363

    Plasmid

    #78984

    Purpose

    255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Inserts

    rHGH
    IFNa2b
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT112_GalL_nCDA1-xBE3

    Plasmid

    #141318

    Purpose

    Expressing base editor nCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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