8,388 results
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Plasmid#145395PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZJOM108
Plasmid#133665PurposeIntegration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:mTREK-1_cryst
Plasmid#133269PurposeP. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFPDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
TagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-GGA2-HaloTagx2
Plasmid#140150PurposePlasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2DepositorInsertGGA2
ExpressionYeastAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pMG
Plasmid#138264PurposeYeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.DepositorInsertseGFP flanked by iCas9 sites
mCherry
UseCRISPRExpressionYeastPromoterTef1Available SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsCERK
Plasmid#118600PurposeExpresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphateDepositorInsertHomo sapiens ceramide kinase
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceFeb. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM12
Plasmid#133638PurposeIntegration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4393
Plasmid#129657PurposeLYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS22H
Plasmid#133904PurposeExpression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWHDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICE MET17
Plasmid#122880PurposeMET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS313-PRP8
Plasmid#89966Purposecomplements prp8 genomic deletionDepositorAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8383
Plasmid#126917PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1DepositorInserttHMG1
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE LEU2
Plasmid#122878PurposeLEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0758
Plasmid#133511PurposeExpression of 3GFP tagged RasAct probe to monitor active Ras1DepositorInsertpLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0543
Plasmid#133493PurposeExpression of nucleo-cytoplasmic mCherry in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0663
Plasmid#133478PurposeExpression in fission yeast from promoter pact1, integrated at his5 locusDepositorInsertpHis5(StuI)-p(act1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0586
Plasmid#133473PurposeIntegration into fission yeast genome at lys3 locus with bsdMX selection cassetteDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0624
Plasmid#133491PurposeExpression of cytoplasmic 3mTagBFP2DepositorInsertpUra4(AfeI)-p(tdh1)-3mTagBFP2-terminator(tdh1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0479
Plasmid#133499PurposeExpression of sfGFP excluded from the nucleus with two NES signalsDepositorInsertpHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only