8,133 results

1. pHUM

   Plasmid

   #64170

   Purpose

   harbours marker genes HIS3, URA3 and MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXP732

   Plasmid

   #46058

   Depositor

   Nancy DaSilva

   Article

   Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-yoPS-CFP2-Kan

   Plasmid

   #44948

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPS-CFP2
   

   Expression

   Yeast

   Available Since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYES2.1-cerMET3

   Plasmid

   #107436

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET3 (MET3 Budding Yeast)
   

   Tags

   V5-His

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYES2.1-cerMET17

   Plasmid

   #107443

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET17 (MET17 Budding Yeast)
   

   Tags

   V5-His

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. E2-Crimson-FKBP(L)

   Plasmid

   #85001

   Purpose

   Expresses E2-Crimson-FKBP(L)

   Depositor

   Benjamin Glick

   Article

   Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

   Insert

   E2-Crimson-FKBP(L)
   

   Expression

   Yeast

   Available Since

   Dec. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBES2

   Plasmid

   #83800

   Purpose

   Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Venus
   

   Expression

   Yeast

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-bleMX6-Purg1-3FLAG

   Plasmid

   #19357

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   urg1 promoter
   

   Expression

   Yeast

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETcon(I-LtrI)

   Plasmid

   #41523

   Depositor

   Andrew Scharenberg

   Insert

   I-LtrI
   

   Tags

   3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB3050(gRNA XII-5)

    Plasmid

    #73292

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSN074

    Plasmid

    #166731

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRf1-SweetTrac1

    Plasmid

    #169083

    Purpose

    Expression of SweetTrac1 biosensor in yeast

    Depositor

    Lily S Cheung

    Article

    Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

    Insert

    SweetTrac1
    

    Expression

    Yeast

    Promoter

    PMA1

    Available Since

    Feb. 4, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDZ514 pURA MET25p PP7-PS-2x-yeGFP

    Plasmid

    #72230

    Purpose

    Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYES2.1-cerMET14

    Plasmid

    #107441

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET14 (MET14 Budding Yeast)
    

    Tags

    V5-His

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-hphMX6-P41nmt1-3FLAG

    Plasmid

    #19349

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Expression

    Yeast

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJZC625

    Plasmid

    #62321

    Purpose

    sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA +1x MS2, Pol II promoter with ribozyme cleavage
    

    Expression

    Yeast

    Promoter

    pAdh

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p42Neo 3-F GW

    Plasmid

    #58558

    Purpose

    Multicopy with G418 resistance marker for triple Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK372

    Plasmid

    #73597

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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