7,935 results

1. pGBW-m4046689

   Plasmid

   #149212

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGFP-ATG13(315)

   Plasmid

   #122072

   Purpose

   Expressing GFP-Atg13

   Depositor

   Daniel Klionsky

   Article

   Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

   Insert

   ATG13
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUG-amdSYM-mak10TR

   Plasmid

   #75433

   Purpose

   Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

   Depositor

   John McCusker

   Inserts

   MAK10TR
   ApR
   amdSYM
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N-terminal 740 bp of MAK10 ORF

   Promoter

   Native promoter and PTEF

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ-pre-pro-alphaf(I)-E2-Crimson

   Plasmid

   #117660

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf(I)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKT0209

   Plasmid

   #8730

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yEGFP
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPICZc-HsACTG1*-thymosinB-8His

   Plasmid

   #111147

   Purpose

   Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   actin gamma 1 (ACTG1 Human)
   

   Use

   Pichia pastoris integration vector

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Mutation

   codon-optimised for expression in Pichia pastoris

   Promoter

   AOX1

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG425GPD-ccdB-DsRed

   Plasmid

   #14370

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. JME4390

   Plasmid

   #129656

   Purpose

   LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJH1260

   Plasmid

   #36913

   Depositor

   Jürgen Heinisch

   Article

   Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

   Inserts

   ScURA3
   ScURA3
   

   Use

   Tags

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB2795

    Plasmid

    #63650

    Purpose

    pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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