8,086 results

1. pRS405 VAC8-RFP

   Plasmid

   #166841

   Purpose

   Expresses Vac8-RFP in yeasts

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FRP1625

   Plasmid

   #64537

   Purpose

   Expresses LexA-ER-B42 in yeast cells

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Insert

   LexA-ER-B42
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ACT1

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGAD-GH-MEFV

   Plasmid

   #92106

   Purpose

   Yeast two hybrid

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   MEFV (MEFV Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCLASP_Crz1(19A)_CLASP_RGS2membrane

   Plasmid

   #133085

   Purpose

   Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   Crz1*-CLASP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pADH1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRC063

   Plasmid

   #123057

   Purpose

   Blue fluorescent protein

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   mTagBFP2 [3]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAW8-aid-V5-Turg1:kanMX6

   Plasmid

   #169357

   Purpose

   C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   1XV5

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG306GAL-ccdB-EYFP

   Plasmid

   #14211

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB2224

   Plasmid

   #67547

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVD_85

   Plasmid

   #149604

   Purpose

   (parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   XylR
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAH2

    Plasmid

    #41826

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7, TEF

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pR208IM1

    Plasmid

    #45351

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

    Insert

    UBR1 mutant
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    D176E

    Promoter

    ADH

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pL59-Nat

    Plasmid

    #140465

    Purpose

    CRISPR/Cas9 engineering with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUDE722

    Plasmid

    #103022

    Purpose

    expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crHCAN1-4.S (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. D588 lys2::HIS3 Disruptor Converter

    Plasmid

    #51675

    Purpose

    Yeast marker swap plasmid for converting LYS2 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046640

    Plasmid

    #153906

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pICE ADE2-URA3

    Plasmid

    #122886

    Purpose

    ADE2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB3041(gRNA X-3)

    Plasmid

    #73283

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDN-T1TCbt

    Plasmid

    #44514

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FRP2002

    Plasmid

    #159311

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mTurquoise2 under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge