8,133 results

1. p416TEF-SEC16

   Plasmid

   #102676

   Purpose

   The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

   Depositor

   Jens Nielsen

   Article

   Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

   Insert

   SEC16 (SEC16 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV0479

   Plasmid

   #133499

   Purpose

   Expression of sfGFP excluded from the nucleus with two NES signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
   

   Expression

   Yeast

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW1673

   Plasmid

   #112047

   Purpose

   CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   SCT1
   

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. YEplac181-TYS1

   Plasmid

   #8629

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics 2004 Mar;166(3):1215-27.

   Insert

   TYS1 (TYS1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   Nov. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJZC572

   Plasmid

   #62318

   Purpose

   sgRNA with 1x com for yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 1x com RNA binding module
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV0543

   Plasmid

   #133493

   Purpose

   Expression of nucleo-cytoplasmic mCherry in P-cells

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
   

   Expression

   Yeast

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG414GAL-ccdB-DsRed

   Plasmid

   #14359

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. JME4393

   Plasmid

   #129657

   Purpose

   LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pITI1

   Plasmid

   #166094

   Purpose

   Gal inducible expression of dCas9-FokI

   Depositor

   Rebecca Burgess

   Insert

   dCas9-FokI
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9-D10A, H840A

   Promoter

   Gal-inducible

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVG48

    Plasmid

    #164706

    Purpose

    rtTA under the control of PGK1 promoter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    PGK1pr - rtTA - CYC1t
    

    Expression

    Yeast

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS424-TUB1-Cterminal6xHis

    Plasmid

    #60387

    Purpose

    Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Tags

    6xHIS

    Expression

    Yeast

    Promoter

    GAL

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pILGFP3

    Plasmid

    #65451

    Purpose

    pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Tags

    yEGFP reporter

    Expression

    Yeast

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pITGFP3

    Plasmid

    #65452

    Purpose

    pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Tags

    yEGFP-CLN2PEST reporter

    Expression

    Yeast

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pWS627

    Plasmid

    #123064

    Purpose

    G___ bicistronic protein

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Ste4-2A-Ste18 [3-4a]
    

    Expression

    Yeast

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDN-G1Tt

    Plasmid

    #44509

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG303GAL-ccdB-EYFP

    Plasmid

    #14205

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046207

    Plasmid

    #145559

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pZHY500

    Plasmid

    #36186

    Depositor

    Daniel Voytas

    Article

    Zhang et al Plant Physiol. 2012 Nov 2.

    Insert

    FokI homodimeric Nuclease domain
    

    Tags

    ACV5

    Expression

    Yeast

    Mutation

    delta152,+63 truncation to TAL backbone

    Promoter

    TEV

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-hphMX6-P3nmt1-3FLAG

    Plasmid

    #19348

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Expression

    Yeast

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSJD001

    Plasmid

    #158589

    Purpose

    Pex22(1-36)-mRuby2 peroxisome marker

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    Pex22(1-36)-mRuby2
    

    Expression

    Yeast

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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