8,060 results

1. pDN-T1GZmbh

   Plasmid

   #44522

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
   yEGFP::ZeoR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Three tandem tet operators (3XtetO2) downstream o…

   Promoter

   PGal1-T123 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Use

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. p414TEF_Hsp82_CycTER

   Plasmid

   #61711

   Purpose

   Yeast plasmid with Hsp82

   Depositor

   Dan Bolon

   Article

   Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

   Insert

   6xHis-Hsp82 (HSP82 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB2192

   Plasmid

   #67539

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCUP1-NMGR

   Plasmid

   #1211

   Depositor

   Susan Lindquist

   Article

   Li et al Science 2000 Jan 28;287(5453):661-4.

   Insert

   sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)
   

   Use

   Tags

   GR

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIplac211-STV1-iGFP

   Plasmid

   #177111

   Purpose

   Expresses Stv1-iGFP in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   STV1 (STV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA-MNase-CaURA3

   Plasmid

   #162461

   Purpose

   DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3

   Depositor

   Adnane Sellam

   Article

   Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

   Insert

   3FLAG-Mnase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA-MNase-CaHIS1

   Plasmid

   #162462

   Purpose

   DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1

   Depositor

   Adnane Sellam

   Article

   Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

   Insert

   3FLAG-Mnase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB3aN_14*

   Plasmid

   #98548

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDSM-bc-Ptdh3-CAD-Ttdh1

    Plasmid

    #127727

    Purpose

    Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptdh3

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTCW008

    Plasmid

    #83526

    Purpose

    pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

    Depositor

    Claudia Vickers

    Article

    Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

    Insert

    pFUS1J2-mfalpha2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHpgRNA50

    Plasmid

    #160128

    Purpose

    To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-LSC2- HDV-TAMO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GAL FLAG46QDProCFPp303

    Plasmid

    #15585

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

    Plasmid

    #72234

    Purpose

    Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    PP7-PS-2x-yeGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADE3p

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNXgate33-3HA

    Plasmid

    #105075

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    3xHA and NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046475

    Plasmid

    #145531

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAG416GAL-ccdB-DsRed

    Plasmid

    #14363

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. TSG101

    Plasmid

    #31412

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Tumor susceptibility gene 101 (TSG101 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pDN-T2dGZmxh

    Plasmid

    #44552

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

    Inserts

    PTETREG promoter
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTETREG

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRTetR-GAL80

    Plasmid

    #185853

    Purpose

    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge