8,173 results
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Plasmid#44514DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only
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pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneTagsyEGFP reporterExpressionYeastAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneTagsyEGFP-CLN2PEST reporterExpressionYeastAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FTKanAX
Plasmid#73900Purposefission yeast drug counter-selectable selection markerDepositorInsertTK-kan
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWS627
Plasmid#123064PurposeG___ bicistronic proteinDepositorInsertSte4-2A-Ste18 [3-4a]
ExpressionYeastAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKT0174
Plasmid#8720DepositorInsertyECFP
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N144I, M153T, V163AAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGAD-GH-MEFV
Plasmid#92106PurposeYeast two hybridDepositorInsertMEFV (MEFV Human)
ExpressionYeastAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3041(gRNA X-3)
Plasmid#73283PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAH2
Plasmid#41826PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7, TEFAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNB813
Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
TagsPEST degronExpressionYeastPromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-GGA2-HaloTagx2
Plasmid#140150PurposePlasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2DepositorInsertGGA2
ExpressionYeastAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUDE722
Plasmid#103022Purposeexpression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)DepositorAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRP1625
Plasmid#64537PurposeExpresses LexA-ER-B42 in yeast cellsDepositorInsertLexA-ER-B42
ExpressionYeastPromoterACT1Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJD001
Plasmid#158589PurposePex22(1-36)-mRuby2 peroxisome markerDepositorInsertPex22(1-36)-mRuby2
ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
D588 lys2::HIS3 Disruptor Converter
Plasmid#51675PurposeYeast marker swap plasmid for converting LYS2 to HIS3DepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVW284
Plasmid#139494PurposeExpression vector for the PP7-GFP protein in S. cerevisiaeDepositorInsertpADH1 PP7∆FG-GFPenvy tCYC1
ExpressionYeastMutation∆FGPromoterpADH1Available SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW300
Plasmid#139495PurposeExpression vector for the PP7-GFP protein in S. cerevisiaeDepositorInsertpTEF PP7∆FG-GFPenvy tCYC1
ExpressionYeastMutation∆FGPromoterpTEFAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsCERK
Plasmid#118600PurposeExpresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphateDepositorInsertHomo sapiens ceramide kinase
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceFeb. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AtCOPT1 upGFP
Plasmid#117899Purposeprotein expressionDepositorAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only