8,133 results

1. pKT-mCitrineV163A-HIS

   Plasmid

   #64685

   Purpose

   pKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.

   Depositor

   Erin O'Shea

   Article

   Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

   Insert

   mCitrine V163A
   

   Expression

   Yeast

   Mutation

   Valine 163 changed to alanine

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Osa-pCAMBIA-1301-STTM166

   Plasmid

   #84197

   Purpose

   Disturb the expression of miR166 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR166
   

   Expression

   Bacterial, Plant, and Yeast…

   Promoter

   2x35S

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pILGFP4L

   Plasmid

   #104507

   Purpose

   Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SmGAL2 promoter-yEGFP
   

   Expression

   Yeast

   Available Since

   Nov. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNB784

   Plasmid

   #60163

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   SIC1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETCON-IgG1/design_50

   Plasmid

   #30301

   Depositor

   David Baker

   Article

   Fleishman et al J Mol Biol. 2011 Sep 29.

   Insert

   IgG Fc Designed Binder IgG1
   

   Expression

   Yeast

   Available Since

   June 8, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pRS DV DNM2-mRUBY2

   Plasmid

   #107793

   Purpose

   donor vector for knock-in gene editing at human DNM2 locus

   Depositor

   Sandra Schmid

   Article

   Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

   Insert

   DNM2-mRuby2 (DNM2 Human, Synthetic)
   

   Use

   Yeast-e.coli shuttle vector

   Expression

   Bacterial and Yeast

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS DV DNM1-EGFP

   Plasmid

   #107794

   Purpose

   donor vector for knock-in gene editing at human DNM1 locus

   Depositor

   Sandra Schmid

   Article

   Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

   Insert

   DNM1-eGFP (DNM1 Human, Synthetic)
   

   Use

   Yeast-e.coli shuttle vector

   Expression

   Bacterial and Yeast

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

   Plasmid

   #178995

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBM4607

   Plasmid

   #18795

   Depositor

   Mark Johnston

   Article

   Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

   Insert

   Sir4 (SIR4 Budding Yeast)
   

   Tags

   myc

   Expression

   Yeast

   Mutation

   aa 951-1200

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046406

    Plasmid

    #153880

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046463

    Plasmid

    #145462

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179033

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Neon(gfp)-Link-TUB1
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pICE TRP1-NatMx

    Plasmid

    #122903

    Purpose

    TRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG303GPD-EGFP-ccdB

    Plasmid

    #14302

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUB23-K-beta-gal

    Plasmid

    #8819

    Depositor

    Alexander Varshavsky

    Article

    Bachmair et al Science 1986 Oct 10;234(4773):179-86.

    Insert

    beta-gal
    

    Tags

    Ubiquitin

    Expression

    Yeast

    Mutation

    K (lysine) at amino terminus of beta-gal

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAG416GPD-EYFP-ccdB

    Plasmid

    #14340

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UASGAL-PCYC

    Plasmid

    #60335

    Purpose

    Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCfB2513

    Plasmid

    #67543

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG426GAL-ccdB-TAP

    Plasmid

    #14275

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 426Gal-FUS-1-501aa-YFP

    Plasmid

    #29601

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-501aa (FUS Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Contains FUS aa# 1-501

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only