8,266 results

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pFA6a-kanMX6-3nmt1-mMaple3

Plasmid

#105167

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

kanMX6-3nmt1-mMaple3

Tags

3nmt1-mMaple3

Expression

Bacterial and Yeast

Promoter

3nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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BB3aN_14*

Plasmid

#98548

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAV0764

Plasmid

#133520

Purpose

Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pHpgRNA50

Plasmid

#160128

Purpose

To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1

Depositor

Yongjin Zhou

Article

Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

Insert

PTEF1-HH-gRNA-LSC2- HDV-TAMO

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-iGFPx6

Plasmid

#105252

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAG303GPD-EGFP-ccdB

Plasmid

#14302

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-ccdB-TAP

Plasmid

#14275

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

TAP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pCfB6679

Plasmid

#106158

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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Ube2d2

Plasmid

#31410

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D2 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pIRTetR-GAL80

Plasmid

#185853

Purpose

Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3A2 (2)

Plasmid

#185852

Purpose

Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179077

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)

Expression

Yeast

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-KanMx

Plasmid

#122902

Purpose

TRP1-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pATP416-pluxO5-ptetO7-crtYBI

Plasmid

#165975

Purpose

Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Inserts

Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

C1281A, G1698A and C66A, C1359T

Promoter

Synthetic promoter (pluxO5), Synthetic promoter (…

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGK415-phlTA2-1E

Plasmid

#165970

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pNC953

Plasmid

#20402

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP-reporter

Expression

Yeast

Mutation

X=UbiE (see schematic) linker=deltak

Available Since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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UASGAL-PCYC

Plasmid

#60335

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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LD111

Plasmid

#69165

Purpose

Integration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg11-EGFP

Tags

EGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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