8,175 results
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Plasmid#145426PurposeYeast Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
p41Neo MagicFluor
Plasmid#58564Purposep41Neo carrying both mating type markers buffered by the Cyc1 terminatorDepositorInsertSte2p-Citrine, Cyc1 term, Ste3p-mCherry
ExpressionYeastAvailable SinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH646-2µ-RLuc
Plasmid#40600DepositorInsertRenilla Luciferase
ExpressionYeastPromoterADH1Available SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB6577
Plasmid#106129PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ377
Plasmid#74214PurposeCombined sgRNA/Cas9 vector with LEU2 markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046632
Plasmid#153929PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpTEF1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD102
Plasmid#69161PurposeIntegration of GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg2-EGFP
TagsEGFPExpressionYeastAvailable SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0662
Plasmid#133477PurposeExpression in fission yeast from promoter pact1, integrated at lys3 locusDepositorInsertpLys3(BstZ17I)-p(act1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
HEL binding scFv D44.1
Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
Tagscmyc tagExpressionYeastAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Num1-PH
Plasmid#71374PurposeFor analysis of PH domain localization in yeastDepositorInsertNum1-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa2563-2692Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4H
Plasmid#104503PurposeUsed to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSaGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4J
Plasmid#104505PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL1 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4N
Plasmid#104509PurposeUsed to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSbGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4R
Plasmid#104513PurposeUsed to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSuGAL2 promoter-yEGFP
ExpressionYeastAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPTK016-3-RFP_Intra
Plasmid#84975PurposeType 3, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable SinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046619
Plasmid#149218PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFTK078
Plasmid#171350PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTxlnA ANIA_03613 terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only