8,086 results

1. pRS426-GFP-Num1-PH

   Plasmid

   #71374

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Promoter

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pWZ414-F53

   Plasmid

   #23077

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 9 to Arginine, H3 K9R

   Promoter

   Available Since

   April 12, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pWZ414-F30

   Plasmid

   #23066

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Histone H3 changed lysine 9 to Glutamine H3 K9Q

   Promoter

   Available Since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pK218TK620T

   Plasmid

   #1192

   Depositor

   Susan Lindquist

   Article

   Patino et al Science 1996 Aug 2;273(5275):622-6.

   Insert

   Hsp104 (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed lysine 218 to threonine and lysine 620 to…

   Promoter

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

   Plasmid

   #179015

   Purpose

   Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLyGo-Kp-2

   Plasmid

   #163144

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LD116

   Plasmid

   #69168

   Purpose

   Integration of GFP tag at Atg16 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg16-GFP
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB5219

   Plasmid

   #106135

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046620

   Plasmid

   #153894

   Purpose

   Yeast expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHES833

    Plasmid

    #90409

    Purpose

    Expresses YFP under control of GAL1 promoter

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pI4-MTU-DO-HIS

    Plasmid

    #160026

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pI2-MTU-DO-HPH

    Plasmid

    #160019

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pI2-MTU-DO-LEU

    Plasmid

    #160017

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PP75

    Plasmid

    #78945

    Purpose

    Supplemental Figure 3. Integration Site 2.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    Integration Site 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046581

    Plasmid

    #149064

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pPS1888

    Plasmid

    #1360

    Depositor

    Pamela Silver

    Article

    Damelin et al Mol Cell 2000 Jan;5(1):133-40.

    Insert

    NLS-EYFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

17. BS-Lys2

    Plasmid

    #69199

    Purpose

    complementation of yeast auxotrophic marker LYS2

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    LYS2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJAID8Z

    Plasmid

    #165053

    Purpose

    Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJAID6A

    Plasmid

    #165046

    Purpose

    Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSJ125

    Plasmid

    #104344

    Purpose

    Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Chen et al Science. 2017 Jan 27;355(6323). pii: 355

    Insert

    MDH2
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge