8,173 results
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Plasmid#154368PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
ExpressionYeastAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE45
Plasmid#139424PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.DepositorInsertmTurquoise1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE44
Plasmid#139423PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.DepositorInsertmTFP1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE43
Plasmid#139422PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.DepositorInsertmMaroon1
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1674
Plasmid#159726PurposeExpress MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MPTLV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1479a3
Plasmid#159725PurposeExpress MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
TagsGal4DBD and MYITV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSJ182
Plasmid#159723PurposeExpress Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCgDepositorInsertGID4 (VID24 Budding Yeast)
UseYeast two-hybrid assayTags3xflag and Gal4ADExpressionYeastMutationremove stop codonPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSJ392
Plasmid#159722PurposeExpress Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCgDepositorInsertGID10 (GID10 Budding Yeast)
UseYeast two-hybrid assayTags3xflag and Gal4ADExpressionYeastMutationremove stop codonPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
TagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1B-mCherry-StreptagII
Plasmid#158744PurposeExpresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-B (KCNK4 Human)
TagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-290 and N-linked glycosylation sites r…PromoterAOX1Available SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 A
Plasmid#129485PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 A (GLO3 Budding Yeast)
ExpressionYeastMutationMutation of serines 389 and 398 to alanineAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 D
Plasmid#129486PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 D (GLO3 Budding Yeast)
ExpressionYeastMutationMutation of serines 389 and 398 to AspAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Met25 Glo3 ΔGRM-ΔC
Plasmid#129487PurposeExpression in S. cerevisiaeDepositorInsertGlo3 ΔGRM ΔC (GLO3 Budding Yeast)
ExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K
Plasmid#112652PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K
ExpressionYeastMutationR59K (GAP-dead)PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K ΔC
Plasmid#112653PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔC
ExpressionYeastMutationR59K, Δ431-493 (deletion of C-terminal amphipathi…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K ΔGRM-ΔC
Plasmid#112654PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K ΔGRM ΔC
ExpressionYeastMutationR59K, Δ376-493 (deletion of Glo3 motif & C-te…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K Δ2x+ve
Plasmid#112655PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K Δ2x+ve
ExpressionYeastMutationR59K, mutations (R223E, K224E, K225E, K233E, K234…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 A
Plasmid#112656PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398A
ExpressionYeastMutationR59K, mutations (S389A, S398A) in Glo3 motif, non…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 R59K S389,398 D
Plasmid#112657PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K S389,398D
ExpressionYeastMutationR59K, mutations (S389D, S398D) in Glo3 motif, pho…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 ΔGRM-ΔC
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
TagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 A
Plasmid#112660PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 S389,398A
TagssfGFPExpressionYeastMutationmutations (S389A, S398A) in Glo3 motif, non-phosp…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
TagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
TagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K
Plasmid#112647PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K
ExpressionYeastMutationR54KPromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K L246D
Plasmid#112648PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K L246D
ExpressionYeastMutationR54K & L246D (in ALPS motif)PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K AxxA
Plasmid#112649PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K AxxA
ExpressionYeastMutationR54K & Alanine substitution of C-terminal δL …PromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 Δ3xF AxxA
Plasmid#112650PurposeExpression S.cerevisiaeDepositorInsertGcs1 Δ3xF AxxA
ExpressionYeastMutationR54K, W304A, W307A, W310A, W349A, F353APromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT140_GalL_cCDA1-VRERBE3
Plasmid#145103PurposeExpressing base editor cCDA1-VRERBE3 in yeast cellsDepositorInsertcCDA1-VRERBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT46_GalL_cCDA1-BE3
Plasmid#145039PurposeExpresses cCDA1-BE3 in yeast cellsDepositorInsertcCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME5000
Plasmid#129661PurposeEYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP265
Plasmid#159301PurposeYeast tagging vector to create gene fusion with yeast codon optimized mTFP1 under KanMX selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP2010
Plasmid#159313PurposeYeast tagging vector to create gene fusion with yeast codon optimized tSapphire under KanMX selectionDepositorTypeEmpty backboneExpressionYeastMutationV2del, L231HAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1931
Plasmid#159306PurposeYeast tagging vector to create gene fusion with yeast codon optimized mRuby2 under KanMX selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC530
Plasmid#135434PurposeYeast integrating plasmid for pTETO1-Venus reporter geneDepositorInsertpTETO1_Venus
ExpressionYeastPromoterpTETO1Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPS1-Venus
Plasmid#133118PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.DepositorInsertpYPS1-YFP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMK2-Venus
Plasmid#133119PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.DepositorInsertpCMK2-YFP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGYP7-Venus
Plasmid#133120PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.DepositorInsertpGYP7-YFP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_NDT1
Plasmid#140593PurposeExpress NDT1 in S. cerevisiaeDepositorInsertNDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
ExpressionYeastPromoterNDT1 promoter and 3'UTRAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_MCART1K91A
Plasmid#140592PurposeExpress MCART1K91A in S. cerevisiaeDepositorInsertSLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
ExpressionYeastMutationcodon-optimized for expression in S. cerevisiae; …PromoterNDT1 promoter and 3'UTRAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C
Plasmid#81239PurposeU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only