7,935 results

1. pFA CUP1p-NuiHA kanMX

   Plasmid

   #131169

   Purpose

   PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   Nui-HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICXC3GH

   Plasmid

   #49023

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG303GPD-ccdB-EGFP

   Plasmid

   #14182

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSUC2::GNSI (NrsR)

   Plasmid

   #121537

   Purpose

   This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.

   Depositor

   Alexey Merz

   Article

   Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

   Inserts

   CYC1 Promoter (CYC1 Budding Yeast)
   Nyv1 Cytosolic Domain (aa 6-230)
   Snc1 Transmembrane Domain (TMD)
   SUC2 CDS
   SUC2 terminator
   NrsR
   

   Use

   Tags

   mGFP5

   Expression

   Yeast

   Mutation

   M109I (Naturally occurring SNP) and The first 21 …

   Promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPGP_scFv

   Plasmid

   #183732

   Purpose

   Expresses anti-lysozyme scFv antibody

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Anti-lysozyme scFv
   

   Use

   Tags

   His6-tag

   Expression

   Yeast

   Mutation

   Promoter

   GAP promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKmK.P3

   Plasmid

   #125036

   Purpose

   Storage plasmid for K.marxianus promoter; type 2 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   ENO1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG426GPD-ccdB-Cerulean

   Plasmid

   #14396

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Cerulean

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPTK006-3a-αMF no EAEA

   Plasmid

   #84965

   Purpose

   Type 3a, α-mating factor no EAEA - secretion signal

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   α-mating factor no EAEA secretion tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Non

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG306-GPD-VPH2 chr I

   Plasmid

   #41897

   Purpose

   Yeast expression of VPH2.

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Insert

   VPH2 (VPH2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Feb. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAFZifPZ431

    Plasmid

    #185849

    Purpose

    Expressing artificial trans-activator in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PHAC1>ZifPZ43>MED3>PABF1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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