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pFA6a-mTID-HA3-KanMX6

Plasmid

#227982

Purpose

To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Expression

Yeast

Promoter

PGK

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHRW40

Plasmid

#130973

Purpose

HyPr plasmid with nptII marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Expression

Yeast

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTAJAK-71 (pESC-NatMXsyn-USER)

Plasmid

#78232

Purpose

Empty vector, for the insertion of gRNA expression cassettes into the vector.

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAG426GPD-Cerulean-ccdB

Plasmid

#14420

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAWH

Plasmid

#133894

Purpose

Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBKWH

Plasmid

#133898

Purpose

Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-A53T

Plasmid

#181722

Purpose

Galactose inducible expression of Alpha-synuclein-A53T

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

A53T

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fCLIP-3xHA

Plasmid

#188936

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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