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8,173 results

Showing: 7721 - 7760 of 8173 results

  6. pNB786

    Plasmid
    #60151
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60151-plasmid-map-sequence-id-96314

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  9. pNB682

    Plasmid
    #60143
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    A4V
    Promoter
    MET17pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60143-plasmid-map-sequence-id-96214

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  13. Gal4pBS134-Pleum

    Plasmid
    #60339
    Purpose
    Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Expression
    Yeast
    Available Since
    Feb. 11, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    60339-plasmid-map-sequence-id-96620

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  20. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

    Plasmid
    #60394
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Synthetic, Budding Yeast)
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

    Plasmid
    #60405
    Purpose
    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB2
    Expression
    Yeast
    Mutation
    TUB2 amino acids 429 - end, replaced with human T…
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. pNB693

    Plasmid
    #60145
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60145-plasmid-map-sequence-id-96228

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  27. p426 3-F GW

    Plasmid
    #58556
    Purpose
    pRS426 carrying Gateway cassette for three inserts
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Nov. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    58556-plasmid-map-sequence-id-90661

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  30. pNB689

    Plasmid
    #60133
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60133-plasmid-map-sequence-id-96189

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  32. pNB752

    Plasmid
    #60155
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    ADE17pr
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60155-plasmid-map-sequence-id-96323

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  35. p41Nat 3-F GW

    Plasmid
    #58553
    Purpose
    CEN vector with clonNat resistance marker for triple Gateway insertion
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    58553-plasmid-map-sequence-id-90657

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  37. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

    Plasmid
    #52898
    Purpose
    YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Ypd*1-L-IFP-F[2]
    Tags
    L-IFP-F[2]
    Expression
    Yeast
    Mutation
    Changed Tryptophane 80 to Alanine
    Promoter
    GAL1
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7721 - 7760 of 8173 results