8,126 results

1. pAG416GPD-Cerulean-YPT1

   Plasmid

   #18848

   Depositor

   Susan Lindquist

   Article

   Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   YPT1 (YPT1 Budding Yeast)
   

   Tags

   Cerulean

   Expression

   Yeast

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

   Plasmid

   #68280

   Purpose

   SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Mkk2DS NLS mCherry (MKK2 Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   RPS2

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXP312

   Plasmid

   #26834

   Depositor

   Suzanne Sandmeyer, Nancy DaSilva

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Available Since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDP010

   Plasmid

   #101166

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pREP4xV5_ccdB

   Plasmid

   #79016

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

   Depositor

   Göran Akusjärvi, Pernilla Bjerling

   Type

   Empty backbone

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB761

   Plasmid

   #60156

   Purpose

   Firefly luciferase (Promega) driven by ADE17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   ADE17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB2991

   Plasmid

   #63643

   Purpose

   pCfB2991 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTG005

   Plasmid

   #71386

   Purpose

   localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

   Depositor

   Mark Schmitt

   Article

   Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

   Insert

   RMP1 (RMP1 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Promoter

   ACT1

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB116

   Plasmid

   #53365

   Purpose

   To express Cup9p‐FLAG from the PGAL1 promoter

   Depositor

   Alexander Varshavsky

   Article

   Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

   Insert

   Cup9 (CUP9 Budding Yeast)
   

   Tags

   Flag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Ube2i

    Plasmid

    #31420

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2I Human)
    

    Tags

    HA

    Expression

    Yeast

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pRS413 Prp5 WT

    Plasmid

    #87599

    Purpose

    Encodes Prp5 WT for expression in yeast

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    PRP5 (PRP5 )
    

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNB789

    Plasmid

    #60166

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    RNR1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG423GAL-ccdB-TAP

    Plasmid

    #14269

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)

    Plasmid

    #14846

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    PHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
    

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    TATA-A1T6. TATA box mutated from TATATAAG to AATA…

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pDS297

    Plasmid

    #34986

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    cpPDZ
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ube2h

    Plasmid

    #31419

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2H Human)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    *see comments

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Ube2L3

    Plasmid

    #31421

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2L3 Human)
    

    Tags

    HA

    Expression

    Yeast

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pGBT9-hSyntenin2b

    Plasmid

    #89422

    Purpose

    Two-hybrid system

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Tags

    GAL4 DBD

    Expression

    Yeast

    Mutation

    * see below

    Promoter

    ADH1

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLUK

    Plasmid

    #64184

    Purpose

    harbours marker genes LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

    Plasmid

    #68274

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS mCherry
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    pRPS2

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge