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PhyB-mCherry-NLS

Plasmid

#51571

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Publication

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

NLS and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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HO-Poly-HO

Plasmid

#51661

Purpose

yeast plasmid for integration of target sequence at HO locus

Depositor

David Stillman

Publication

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p413TEF-SirT1

Plasmid

#51746

Purpose

overexpression of human SirT1 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Publication

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

SirT1 (SIRT1 Human)

Expression

Yeast

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEV-G4-Ph

Plasmid

#46820

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTH728-CEN-RLuc/maxCFLuc

Plasmid

#38212

Depositor

Tobias von der Haar

Publication

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS

Plasmid

#29591

Depositor

Aaron Gitler

Publication

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Expression

Yeast

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLIPglu-30μΔ13V

Plasmid

#27998

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-30μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

D154A in mglB

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLIPglu-170nΔ13V

Plasmid

#27999

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-170nΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

WT binding protein (no F16A)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pRS416 Gal G294A YFP

Plasmid

#27448

Depositor

Aaron Gitler

Publication

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Publication

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Expression

Yeast

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-T7-kanMX6

Plasmid

#20760

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-P3nmt1-3FLAG

Plasmid

#19345

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

natMX6

Expression

Yeast

Available since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-natMX6-P41nmt1-3FLAG

Plasmid

#19346

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

natMX6

Expression

Yeast

Available since

Sept. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pAG304GAL-ccdB

Plasmid

#14135

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSC-humanADAR1

Plasmid

#198235

Purpose

Expresses human ADAR1 in yeast

Depositor

Brenda Bass

Publication

Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

Insert

human ADAR1 (ADAR Human)

Tags

His10_TEV

Expression

Yeast

Promoter

GAL1

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGJJ045

Plasmid

#183621

Purpose

abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

Depositor

Ben Lehner

Publication

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Publication

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-sfGFP-kanMX (pMaM17)

Plasmid

#173452

Purpose

Contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

Insert

mCherry-sfGFP-ADH1ter

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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