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YCplac33-NAT_MCART1

Plasmid

#140591

Purpose

Express MCART1 in S. cerevisiae

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)

Use

Tags

Expression

Yeast

Mutation

codon-optimized for expression in S. cerevisiae

Promoter

NDT1 promoter and 3'UTR

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pKL07

Plasmid

#104431

Purpose

mitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

mito-Super Ecliptic pHluorin-mRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Tef1

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pVB3011

Plasmid

#113389

Purpose

Galactose-inducible L-BC gag: cure killer

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

L-BC Gag

Use

Tags

Expression

Yeast

Mutation

Please see depositor comments

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Use

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS306-URA3-PHIS3-PfV-GS-Sapphire

Plasmid

#116932

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAD-IRP

Plasmid

#220751

Purpose

Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Insert

Iron Regulatory Protein (ACO1 )

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGADCg

Plasmid

#20161

Depositor

Peter Uetz

Article

Stellberger et al Proteome Sci. 2010 . 8():8.

Type

Empty backbone

Use

Tags

Gal4 AD and SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pXNubA22-Dest

Plasmid

#105087

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

Type

Empty backbone

Use

Tags

NubA

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-hphMX6

Plasmid

#105156

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

mCherry-hphMX6

Use

Tags

mCherry

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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