8,086 results

1. pAG424-10X-tRNA

   Plasmid

   #70125

   Purpose

   Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Insert

   10X-tRNA block
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   None – SUP4 pol III promoter provided by insert

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

2. Ube2b

   Plasmid

   #31408

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2B Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pZ_P-GAP-eGFP

   Plasmid

   #126717

   Purpose

   yeast plasmid overexpressing eGFP

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   egfp
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pILGFP3AG4

   Plasmid

   #185874

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYES2-HTH-HHT2

   Plasmid

   #86482

   Purpose

   yeast expression of HHT2 with His10-TEV-HA tag

   Depositor

   Akash Gunjan

   Article

   Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

   Insert

   HHT2 (HHT2 Budding Yeast)
   

   Use

   Tags

   HIS10-TEV-HA (HTH)

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Jan. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Ube2r1

   Plasmid

   #31426

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (CDC34 Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. Ube2s

   Plasmid

   #31417

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBA7 Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pWS172

   Plasmid

   #90519

   Purpose

   Cas9 gap repair vector - HIS3

   Depositor

   Tom Ellis

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ube2t

   Plasmid

   #31427

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2T Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

10. ZJOM75

    Plasmid

    #133649

    Purpose

    Integration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ANP1-mCherry (ANP1 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCTCON2-GFP

    Plasmid

    #158130

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104908

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH124

    Plasmid

    #102951

    Purpose

    Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

    Depositor

    Peter Espenshade

    Article

    Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

    Insert

    APEX2-NES
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG303-Gal-GR100

    Plasmid

    #84908

    Purpose

    induce expression of dipeptide repeat GR in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    GR100 (C9orf72 Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-tdTomato-hphMX6

    Plasmid

    #105157

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

    Insert

    tdTomato-hphMX6
    

    Use

    Tags

    tdTomato

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY2-MET-IV

    Plasmid

    #218117

    Purpose

    MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY2-LYS-IV

    Plasmid

    #218118

    Purpose

    LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS426-GFP-COF1

    Plasmid

    #31844

    Depositor

    David Drubin

    Article

    Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

    Insert

    Cofilin (COF1 Budding Yeast)
    

    Use

    Tags

    GFP (S65T)

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available Since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pWS171

    Plasmid

    #90518

    Purpose

    Cas9 gap repair vector - LEU2

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR

    Plasmid

    #24585

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available Since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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