8,016 results

1. pMEL17

   Plasmid

   #107923

   Purpose

   TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PP74

   Plasmid

   #78944

   Purpose

   Supplemental Figure 3. Integration Site 1.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   Integration Site 1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDRf1-4CL5-AtSCT (JBEI-11557)

   Plasmid

   #87937

   Purpose

   Co-expression in S.cerevisiae of 4CL5 and AtSCT

   Depositor

   Dominique Loque

   Article

   Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

   Inserts

   AtSCT
   4CL5 (4CL5 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYES2.1-pomSua1

   Plasmid

   #107446

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Sua1
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   See depositor comments below.

   Promoter

   GAL1

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYES2.1-pomMet14

   Plasmid

   #107451

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met14 (met14 Fission Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYES2.1-pomSir1

   Plasmid

   #107447

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Sir1 (sir1 Fission Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   H523R, A750T

   Promoter

   GAL1

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYES2.1-pomMet1

   Plasmid

   #107444

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met1
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-SKII-3XHA-HALO-Phleomycin

   Plasmid

   #188930

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-Phleomycin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDRFLIP37

   Plasmid

   #65515

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-Cit-attR1 and attR2-Cer-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS423-mitoT

    Plasmid

    #174525

    Purpose

    Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    Yeast mitochondrial intermembrane tether
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p42Nat 1-F GW

    Plasmid

    #58549

    Purpose

    Multicopy with clonNat resistance marker for single Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST-N2H-F2-empty vector

    Plasmid

    #125552

    Purpose

    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FRP1999

    Plasmid

    #159308

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCUP1pNuiHA kanMX CEN

    Plasmid

    #131168

    Purpose

    for ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiae

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    Nui-HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KM-CashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174838

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJZC518

    Plasmid

    #62277

    Purpose

    Yeast dCas9 expression plasmid

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Has mutations in the RuvC1 and HNH domains to ren…

    Promoter

    pTdh3

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSX6

    Plasmid

    #90468

    Purpose

    U6 wt in pSE358 (TRP1 CEN)

    Depositor

    Christine Guthrie

    Article

    Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

    Insert

    snR6 (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    native

    Available since

    June 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMEL13

    Plasmid

    #107919

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB6638

    Plasmid

    #106165

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMTU-DO-HIS-C4

    Plasmid

    #160340

    Purpose

    High-copy expression vector for K. marxianus, histidine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPGP_scFv

    Plasmid

    #183732

    Purpose

    Expresses anti-lysozyme scFv antibody

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Use

    Tags

    His6-tag

    Expression

    Yeast

    Mutation

    Promoter

    GAP promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS405 VAC8-GFP

    Plasmid

    #166836

    Purpose

    Expresses Vac8-GFP in yeasts.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 416Gal-FUS-R521G-YFP

    Plasmid

    #29623

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R521G

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pGK401red-hENT1

    Plasmid

    #166028

    Purpose

    Expresses human equilibrative nucleoside transporter 1 (hENT1)

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    human equilibrative nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB6627

    Plasmid

    #106159

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAFZifPZ431

    Plasmid

    #185849

    Purpose

    Expressing artificial trans-activator in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PHAC1>ZifPZ43>MED3>PABF1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMJB1-RPS22B-Cl

    Plasmid

    #160431

    Purpose

    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPS22B (RPS22B Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to change nucleo…

    Promoter

    GAL1 and S. cerevisiae GAL1

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDRFLIP36

    Plasmid

    #65514

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-Cer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGRCRE

    Plasmid

    #129116

    Purpose

    Expresses cre recombinase in Z. rouxii yeast cells

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Insert

    cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    S. cerevisiae GAL1 promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GAL FLAG25QDProCFPp303

    Plasmid

    #15584

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

31. pMG13

    Plasmid

    #122932

    Purpose

    Expression of the regulator tTA.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    tTA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pcln3

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pREP42-MCS+

    Plasmid

    #52691

    Purpose

    Same as pREP42, with better MCS

    Depositor

    Michael Nick Boddy

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pnmt41

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mito-yHS1-M7A

    Plasmid

    #159164

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of mito-yHS1…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. YIplac211-HaloTag-DRS2

    Plasmid

    #177103

    Purpose

    Expresses HaloTag-Drs2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    DRS2 (DRS2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Use

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-ytdTomato-CaURA3

    Plasmid

    #118461

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PP255

    Plasmid

    #78934

    Purpose

    Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p404CYC1

    Plasmid

    #15971

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    CYC1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFTK004

    Plasmid

    #171276

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    PgpdA ANIA_08041 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG426GPD-EYFP-ccdB

    Plasmid

    #14348

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge