7,933 results

1. pHES875

   Plasmid

   #89192

   Purpose

   Expresses mCherry under control of GAL1 promoter.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pGAL1-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETCON-TNF

   Plasmid

   #67748

   Purpose

   Tumor Necrosis Factor Alpha Yeast Display Plasmid

   Depositor

   Timothy Whitehead

   Article

   Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

   Insert

   TNF-Alpha
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG306-pTet07-synYFP[TTG/AGA]-nLuc

   Plasmid

   #199756

   Purpose

   Elongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stalling

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   synYFP[TTG/AGA]-nLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pESC-URA-hPOLISc

   Plasmid

   #60819

   Purpose

   to express human Pol iota recoded to yeast codons in yeast

   Depositor

   Youri Pavlov

   Article

   Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

   Insert

   POLI (POLI Human)
   

   Use

   Tags

   c-myc

   Expression

   Yeast

   Mutation

   optimized to yeast codon usage

   Promoter

   GAL1

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046479

   Plasmid

   #145495

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pJZC518

   Plasmid

   #62277

   Purpose

   Yeast dCas9 expression plasmid

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Has mutations in the RuvC1 and HNH domains to ren…

   Promoter

   pTdh3

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p404CYC1

   Plasmid

   #15971

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   CYC1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFTK004

   Plasmid

   #171276

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PgpdA ANIA_08041 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDRFLIP42

   Plasmid

   #65518

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-Cit-attR1 and attR2-mCer-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDRFLIP35

    Plasmid

    #65513

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-TFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRFLIP32

    Plasmid

    #65511

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYES2.1-cerMET6

    Plasmid

    #107438

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET6 (MET6 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. D771 leu2::LYS2 Disruptor Converter

    Plasmid

    #51677

    Purpose

    Yeast marker swap plasmid for converting LEU2 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA CRU HISMX

    Plasmid

    #131164

    Purpose

    PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    CRU

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA NuiHA-loxP-nat-CUP1p

    Plasmid

    #131170

    Purpose

    PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    Nui-HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Use

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GAL FLAG25QDProCFPp303

    Plasmid

    #15584

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. YIplac211-NHX1-HaloTagx2

    Plasmid

    #177107

    Purpose

    Expresses Nhx1-HaloTagx2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    NHX1 (NHX1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-ytdTomato-CaURA3

    Plasmid

    #118461

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSB305-K3L-vac

    Plasmid

    #20053

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2008 Nov 30. ():.

    Insert

    K3L
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCfB3035(X-4 MarkerFree)

    Plasmid

    #73273

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJK148-Pnmt41-GBP-mCherry(C)-leu1+

    Plasmid

    #89069

    Purpose

    genomic GBP-mCherry epitope C-terminal tagging at leu1+ locus in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Genomic integration in s. pombe

    Tags

    GBP-mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YIplac211-ANP1-msGFP2

    Plasmid

    #177100

    Purpose

    Expresses Anp1-msGFP2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    ANP1 (ANP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEDJ400

    Plasmid

    #177269

    Purpose

    USER cloning-compatible 2μ vector (URA3)

    Depositor

    Michael Jensen

    Article

    Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKT0150

    Plasmid

    #8741

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yESapphire
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with S72A, Y145F, T203I

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pREP42-MCS+

    Plasmid

    #52691

    Purpose

    Same as pREP42, with better MCS

    Depositor

    Michael Nick Boddy

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pnmt41

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GAL FLAG46QDProGFP p306

    Plasmid

    #15832

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pDA183

    Plasmid

    #74247

    Purpose

    pSTL1-dPSTRr(2,1)

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    pSTL1-dPSTRr(2,1)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG426GAL-ccdB-EYFP

    Plasmid

    #14227

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG416GALL-ccdB-6stop

    Plasmid

    #203511

    Purpose

    Gateway-compatible destination vector for yeast expression with GALL promoter/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAG416GAL-3x-uC-ccdB-6stop

    Plasmid

    #203514

    Purpose

    Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG416GAL10-ccdB-6stop

    Plasmid

    #203512

    Purpose

    Gateway-compatible destination vector for yeast expression with GAL10 promoter/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAG416GAL-1x-ccdB-6stop

    Plasmid

    #203519

    Purpose

    Gateway-compatible destination vector for yeast expression with GAL1 promoter (1x GAL4 binding sites)/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG416GAL-2x-uA-ccdB-6stop

    Plasmid

    #203517

    Purpose

    Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG416GAL-DENV_NS3-GS-NS2B

    Plasmid

    #201940

    Purpose

    Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    DENV NS3-GS-NS2B (POLY )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAG416GALL-HIV-1_PR

    Plasmid

    #203493

    Purpose

    Expresses HIV-1 protease from a GALL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    HIV-1 protease
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GALL

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAG416GAL10-WNV_NS2B-GS-NS3

    Plasmid

    #203475

    Purpose

    Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    WNV NS2B-GS-NS3 (POLY )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG426GAL-SARS-CoV-2_PLP

    Plasmid

    #203509

    Purpose

    Expresses SARS-CoV-2 papain-like protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    SARS-CoV-2 PLP (ORF1ab )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAG426GAL-KUNV_NS2B-GS-NS3

    Plasmid

    #203506

    Purpose

    Expresses KUNV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    KUNV NS2B-GS-NS3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAV123

    Plasmid

    #63189

    Purpose

    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge