8,418 results
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Plasmid#99538PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 markerDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEGFP-KanMX6
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E2-Crimson-FKBP(M)
Plasmid#85002PurposeExpresses E2-Crimson-FKBP(M)DepositorInsertE2-Crimson-FKBP(M)
ExpressionYeastAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3051(gRNA X-3 XI-2 XII-2)
Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfB3045(gRNA XI-3)
Plasmid#73287PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
ExpressionBacterial and YeastPromoterpombe TEFAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS406-CMV-lacI-3FLAG
Plasmid#83410PurposeExpression of lacI in yeastDepositorInsertlacI repressor
Tags3xFLAG and NLSExpressionYeastPromoterCMVAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
ExpressionYeastPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS35
Plasmid#83797PurposeUseful for tagging yeast proteins with C-terminal mCherry tag; hygromycin-B selectionDepositorPublicationInsertmCherry
TagsmCherryExpressionYeastAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHLU
Plasmid#64167Purposeharbours marker genes HIS3, LEU2 and URA3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAV123
Plasmid#63189PurposepRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PP74
Plasmid#78944PurposeSupplemental Figure 3. Integration Site 1.DepositorInsertIntegration Site 1
ExpressionYeastAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415GPD-3xHA-GW
Plasmid#63781Purposeyeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insertDepositorTypeEmpty backboneTags3xHAExpressionYeastPromoterGPDAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS416gT-GalL-Cas9
Plasmid#79903PurposepRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expressionDepositorInsertsCas9
guide RNA (gRNA)
Tetracycline Repressor
UseCRISPRExpressionYeastPromoterGPM1 promoter, GalL, and RPR1 promoter with TetO …Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD8A-RGR-r10
Plasmid#74370PurposeThis plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorPublicationInsertRGR-r10
UseCRISPR and Synthetic BiologyExpressionYeastPromoterADH1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD6-pGRR-r11-r10-yeGFP
Plasmid#74368PurposepGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.DepositorPublicationInsertyeGFP
UseCRISPR and Synthetic BiologyExpressionYeastPromoterpGRR-r11-r10Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only