8,086 results

1. pYEXNH3

   Plasmid

   #49024

   Purpose

   FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYES2.1-pomMet8

   Plasmid

   #107449

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   Met8
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BB3nK_ext_AD

   Plasmid

   #108687

   Purpose

   Plasmid for assembly of donor DNA templates consisting of three expression units

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p416 25Q GAL

   Plasmid

   #1185

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRR-AR-5Z

   Plasmid

   #23058

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   AR (AR Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pRS315e_pGal-dCas9-PmCDA1

   Plasmid

   #79615

   Purpose

   Expresses dCas9-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-kanMX6-P41nmt1-3FLAG

   Plasmid

   #19337

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   3FLAG
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. PP244

   Plasmid

   #78978

   Purpose

   Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRG659_lexO-FseI_LexA-TF_LEU2MX

    Plasmid

    #154816

    Purpose

    Inducible expression of FseI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    FseI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKG-GW1

    Plasmid

    #203163

    Purpose

    Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

    Plasmid

    #24584

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPTK009-3a-αAmylase-αMFΔ

    Plasmid

    #84968

    Purpose

    Type 3a, α-Amylase followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-Amylase followed by MFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYZ301

    Plasmid

    #102690

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-kanMX6-P81nmt1-3FLAG

    Plasmid

    #19338

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    3FLAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYX233

    Plasmid

    #163762

    Purpose

    Empty vector for Gal-inducible yeast expression

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Type

    Empty backbone

    Use

    Tags

    Hemagglutinin (HA) tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)

    Plasmid

    #199763

    Purpose

    Elongation reporter construct to quantify elongation of LacZ

    Depositor

    Brian Zid

    Article

    Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

    Insert

    LacZ-nLucPEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKT127-SEP

    Plasmid

    #41899

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Type

    Empty backbone

    Use

    Tags

    superecliptic GFP (SEP)

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDRF1-GW ymTurquoise2

    Plasmid

    #118453

    Purpose

    Overexpression of yeast codon-optimized mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIM691

    Plasmid

    #74641

    Purpose

    alias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    ymTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge