8,432 results

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pPS2035

Plasmid

#1368

Depositor

Pamela Silver

Publication

Brodsky et al RNA 2000 Dec;6(12):1737-49.

Insert

U1A (1-94) (RNU1-4 Human)

Tags

GFP

Expression

Yeast

Mutation

U1A (1-94)

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pPS1888

Plasmid

#1360

Depositor

Pamela Silver

Publication

Damelin et al Mol Cell 2000 Jan;5(1):133-40.

Insert

NLS-EYFP

Expression

Yeast

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pG1-NMGR526

Plasmid

#1122

Depositor

Susan Lindquist

Publication

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)

Tags

GR526

Expression

Yeast

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only
5538 pUG2-SSA1

Plasmid

#1247

Depositor

Susan Lindquist

Publication

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Ssa1 (SSA1 Budding Yeast)

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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Lifeact-3xmNG::HIS3

Plasmid

#227596

Purpose

Integration vector for expressing Lifeact-3xmNeonGreen

Depositor

Bruce Goode

Publication

Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

Insert

Lifeact-3xmNeonGreen::HIS3

Tags

3xmNeonGreen

Expression

Yeast

Mutation

Truncated ABP140 (aa 1-17 only)

Promoter

Abp140

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDIV270

Plasmid

#226181

Purpose

Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Publication

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDB5321

Plasmid

#204831

Purpose

An integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Publication

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW3 promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDB5319

Plasmid

#204829

Purpose

An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Publication

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Expression

Yeast

Promoter

CMV promoter and enotetSW1 promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pABY-T2

Plasmid

#201765

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pND003

Plasmid

#200949

Purpose

Yeast surface display destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Publication

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Yeast surface display

Tags

AGA2, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-nLuc

Plasmid

#199755

Purpose

Control construct of elongation reporter to quantify elongation of YFP

Depositor

Brian Zid

Publication

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

Nanoluc

Expression

Yeast

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Publication

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Expression

Yeast

Promoter

tetO7 promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Km ymNeongreen

Plasmid

#193959

Purpose

TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

Depositor

Bas Teusink

Insert

ymNeonGreen

Expression

Yeast

Promoter

TEF1

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_101

Plasmid

#160541

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Publication

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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p413TEF1-ATR1(pYL573)

Plasmid

#178288

Purpose

Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

ATR1

Expression

Yeast

Promoter

TEF1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP8H

Plasmid

#188062

Purpose

PROSS-designed versatile peroxidase 8H

Depositor

Sarel Fleishman

Publication

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3AA5

Plasmid

#185875

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~47 copy)

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK-DN5

Plasmid

#180286

Purpose

NatR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Publication

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - NatR - CEN6/ARS4

Expression

Bacterial and Yeast

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV515

Plasmid

#177703

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
tRNA-sgRNA-tRNA

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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