8,057 results

1. pAV0661

   Plasmid

   #133476

   Purpose

   Expression in fission yeast from promoter pact1, integrated at ade6 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDIS3

   Plasmid

   #92173

   Purpose

   Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

   Depositor

   Judith Berman

   Article

   Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

   Insert

   NAT1
   

   Use

   Genbank: u03440.1

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIplac211-CHS3-msGFPx3

   Plasmid

   #105257

   Purpose

   Plasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Chs3 portion (CHS3 Budding Yeast)
   

   Use

   Tags

   msGFPx3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYZ157

   Plasmid

   #98413

   Purpose

   Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. YIplac211-Vph1-HaloTag

   Plasmid

   #140149

   Purpose

   Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag

   Depositor

   Benjamin Glick

   Article

   Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

   Insert

   VPH1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p112A1NE

   Plasmid

   #85983

   Purpose

   For construction of cDNA expression library.

   Depositor

   Wolf Frommer

   Article

   Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Adh1

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRoC30_Th3.14

   Plasmid

   #188068

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRMH124-VP16-CIB1

   Plasmid

   #40615

   Purpose

   Expresses VP16-CIB1 for light-inducible protein expression when coupled with GalBD-CRY2PHR or GalBD-CRY2

   Depositor

   Chandra Tucker

   Article

   Hughes et al Methods. 2012 Aug 15.

   Insert

   VP16-CIB1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLexA

    Plasmid

    #11342

    Depositor

    Guy Caldwell

    Type

    Empty backbone

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pILGFPE9

    Plasmid

    #83545

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAL513

    Plasmid

    #169153

    Purpose

    Encodes OptoQ-INVRT1

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRR-GR-5Z

    Plasmid

    #23060

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    GR (NR3C1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pBS-SKII-URA-GAL1-fSNAP-3xHA

    Plasmid

    #188935

    Purpose

    Integration cassette to create N-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    URA-GAL1-fSNAP-3xHA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRF1-GW yosfGFP-T2A-mCherry

    Plasmid

    #118448

    Purpose

    Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yosfGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVG122

    Plasmid

    #211765

    Purpose

    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    strongLOV
    

    Use

    For single-copy integration (if cut with pmei)

    Tags

    SV40 NLS and VP16

    Expression

    Yeast

    Mutation

    compared to EL222, strongLOV contains Glu84Asp mu…

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS267

    Plasmid

    #122380

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW1664

    Plasmid

    #112038

    Purpose

    CEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HIS

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    Su9
    

    Use

    Tags

    TagBFP

    Expression

    Yeast

    Mutation

    amino acids 1-76

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. YEp181-CUP1-His-Smt3

    Plasmid

    #99536

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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