8,431 results
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Plasmid#185875PurposeA plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~47 copy)DepositorTypeEmpty backboneExpressionYeastMutationNoneAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only
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pYTK-DN5
Plasmid#180286PurposeNatR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - NatR - CEN6/ARS4
ExpressionBacterial and YeastAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV515
Plasmid#177703PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LT1_34_sfpHluorin
Plasmid#177707PurposePlasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)DepositorInsertpTEF1-sfpHluorin
UseUsed as donor in genome integration after lineari…ExpressionYeastAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-N (LEU2)
Plasmid#177798PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneExpressionYeastPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38-AtLEA4-5
Plasmid#178189PurposeSED1 biosensor for expression in Saccharomyces cerevisiaeDepositorInsertSED1 biosensor
ExpressionYeastPromoterpPMA1Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)
Plasmid#173454PurposeContains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
ExpressionYeastPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-ymVenus-URA3
Plasmid#168058PurposeReplaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymVenus
TagsymVenusExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-ptetO7-pphlO6-crtYBI
Plasmid#165976PurposeExpresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyExpressionYeastMutationC66A, C1359T and C1281A, G1698APromoterSynthetic promoter (pphlO6), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP
Plasmid#158130PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)DepositorInsertsfGFP
ExpressionYeastPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
ExpressionYeastAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-URA
Plasmid#160004PurposeLow-copy expression vector for K. marxianus, uracil selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsn/aExpressionYeastPromotern/aAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS9
Plasmid#139416PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.DepositorInsertmTurquoise2
ExpressionYeastPromoterTEF1Available sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
yHS1-M7A
Plasmid#159151PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A
ExpressionYeastMutationMutated Met 7 of the cyt b562 module of yHS1 to A…PromoterGPDAvailable sinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2BprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWKS5
Plasmid#139412PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.DepositorInsertCitrine
ExpressionYeastPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHRW32
Plasmid#130972PurposeHyPr plasmid with ble markerDepositorInsertempty backbone
ExpressionYeastAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM40
Plasmid#133650PurposeIntegration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM44
Plasmid#133653PurposeIntegration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only