8,223 results

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pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

Plasmid

#179004

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Tags

NLS

Expression

Yeast

Promoter

TEF

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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POM43-mTFP1

Plasmid

#69853

Purpose

N-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf-Btl2

Plasmid

#117664

Purpose

Study secretion efficiency of Btl2

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf-Btl2

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-ura4MX6

Plasmid

#49184

Purpose

gene deletion in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoSuperfolderGFP-CaURA3

Plasmid

#44873

Purpose

Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoSuperfolderGFP

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGK401red-rtetTAK8N, L131L

Plasmid

#165968

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRG207

Plasmid

#64530

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMY.PRC2

Plasmid

#234582

Purpose

Expression of ProtA-EZH2, SUZ12, EED and RBBP4

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Yeast

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAV0532

Plasmid

#133502

Purpose

Expression of mTagBFP2 targeted to the nucleus with one NLS signals

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGAN230

Plasmid

#131122

Purpose

reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdTomato

Plasmid

#118456

Purpose

Overexpression of yeast codon-optimized tdTomato

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-SLEV_NS2B-GS-NS3

Plasmid

#203510

Purpose

Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

poly SLEV NS2B-GS-NS3 (POLY )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-ubiquitin

Plasmid

#196999

Purpose

Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

ubiquitin (UBC Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pNKY51

Plasmid

#14839

Depositor

Nancy Kleckner

Article

Alani et al Genetics. 1987 Aug . 116(4):541-5.

Insert

hisG-URA3-hisG

Expression

Yeast

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pRG215

Plasmid

#64525

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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3410 pBM272 Bcl-XL

Plasmid

#8772

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Expression

Yeast

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only