-
Plasmid#74644PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoterMET25Available sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS
Plasmid#64746PurposeYeast expression plasmid: ACC1 (ser1157ala)DepositorInsertACC1 (ser1157ala)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo
Plasmid#115421PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-msGFP-VPS21
Plasmid#105265PurposePlasmid for pop-in/pop-out replacement of Vps21 with msGFP-Vps21.DepositorInsertVps21 (VPS21 Budding Yeast)
UseTagsmsGFPExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046619
Plasmid#149218PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046219
Plasmid#153926PurposeYeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS42K_PCP-VP64_B-PP7
Plasmid#177157PurposeExpresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cellsDepositorInsertsPCP-VP64
B-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Hsh155 WT
Plasmid#87219PurposePlasmid used to maintain viability after deletion of genomic copy of HSH155.DepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH646-2µ-RLuc
Plasmid#40600DepositorInsertRenilla Luciferase
UseTagsExpressionYeastMutationPromoterADH1Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pV1386
Plasmid#111445PurposeSolo vector pV1382 + sgScADE2DepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314-hUI
Plasmid#11006DepositorInserthUI recombination system
UseTagsExpressionBacterial and YeastMutationInverted repeat recombination system. Recombinati…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046217
Plasmid#149071PurposeYeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJW1670
Plasmid#112044PurposeCEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URADepositorInsertOLE1
UseTagsV5-TEV-EGFPExpressionYeastMutationP319TPromoterGAL1Available sinceMay 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB5219
Plasmid#106135PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPSG465
Plasmid#158587PurposeEnzymes Bv-CYP76AD5, DODC, ARO4_K229LDepositorInsertsBv-CYP76AD5
DODC
ARO4_K229L
UseTagsExpressionYeastMutationK229LPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CHL1 wt-eGFP
Plasmid#117876Purposeprotein expressionDepositorInsertCHL1 wt-eGFP (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAOXAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZmMATE2
Plasmid#117094Purposeprotein expression in yeastDepositorInsertZmMATE2
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
OsMATE
Plasmid#117093Purposeprotein expression in yeastDepositorInsertAtTPT (ORF-HIS)
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtFRD3
Plasmid#117086Purposeprotein expression in yeastDepositorInsertAtFRD3
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXP522
Plasmid#26847DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
plIVMD164
Plasmid#160936PurposeIn vivo mRNA display construct for GFPDepositorInsertMCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LD102
Plasmid#69161PurposeIntegration of GFP tag at Atg2 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg2-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKT0101
Plasmid#8721DepositorInsertyECFP
UseTagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163APromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046620
Plasmid#153894PurposeYeast expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPTK016-3-RFP_Intra
Plasmid#84975PurposeType 3, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ACT1_intron
Plasmid#127602Purposelow-copy URA-selectable plasmid, ACT1 intron inserted into URA3DepositorInsertACT1 intron
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pGBW-m4046581
Plasmid#149064PurposeYeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p41Neo MagicFluor
Plasmid#58564Purposep41Neo carrying both mating type markers buffered by the Cyc1 terminatorDepositorInsertSte2p-Citrine, Cyc1 term, Ste3p-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry
Plasmid#164917PurposeDivergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizerDepositorInsertP(PGK1)-citrine and P(HIS4)-mCherry
UseTagsExpressionYeastMutationPromoterPGK1 (yeast) and HIS4 (yeast)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
ymScarletI-YPET pDRF1-GW
Plasmid#168063PurposeFRET fusion between ymScarletI and YPET for use in yeastDepositorInsertymScarletI-YPET
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 G294A
Plasmid#27459DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pK218TK620T
Plasmid#1192DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationchanged lysine 218 to threonine and lysine 620 to…PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046445
Plasmid#145493PurposeYeast Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046589
Plasmid#145450PurposeYeast Expression plasmid for SARS-CoV-2 ORF6 proteinDepositorInsertSARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-GBP-RFP-kanMX6
Plasmid#105160PurposeYeast gene targetingDepositorInsertGBP-RFP-kanMX6
UseTagsGBP-RFPExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
104b-U1(WT)
Plasmid#1153DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsGAL1:10 promoterExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMZ377
Plasmid#74214PurposeCombined sgRNA/Cas9 vector with LEU2 markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB4782
Plasmid#106141PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6681
Plasmid#106157PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only