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Plasmid#149147PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDN-G1GZmbh
Plasmid#44516DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJA053
Plasmid#128515PurposeExpresses SFA8 in PichiaDepositorInsertSFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)
UseTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3YFP-kanMX6
Plasmid#105149PurposeYeast gene targetingDepositorInsert3YFP-kanMX6
UseTags3YFPExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCH001
Plasmid#47953PurposetetO-GFP reporter casetteDepositorInsertGFP
UseTet inducible expressionTagsExpressionYeastMutationPromoterT3Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
GTL-g
Plasmid#81099PurposeGene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPS1888
Plasmid#1360DepositorInsertNLS-EYFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-EYFP-ccdB
Plasmid#14347DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_HIS3_II
Plasmid#109059Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.DepositorInsertHIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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p413TEF-HST2
Plasmid#51745Purposeoverexpression of HST2 in S. cerevisiae, HIS3 markerDepositorInsertHST2 (HST2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVEG17*
Plasmid#111856PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)DepositorInsertGAL1p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyTagsExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL1pAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
yCp50-PRP2
Plasmid#111304PurposePrp2 in yCp50 plasmidDepositorInsertPRP2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRG711_lexO-AscI_LexA-TF_HIS3MX
Plasmid#154820PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS-1-413aa-YFP
Plasmid#29599DepositorInsertFUS-1-413aa (FUS Human)
UseTagsYFPExpressionYeastMutationContains FUS aa# 1-413PromoterAvailable sinceJuly 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCK011
Plasmid#105343PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK012
Plasmid#105344PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LD229
Plasmid#69192PurposeIntegration of 2GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg29-2EGFP
UseTags2xEGFPExpressionYeastMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mScarletI pDRF1-GW
Plasmid#168085PurposeExpresses Lifeact-mScarletI to visualize actin in yeastDepositorInsertLifeAct-mScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-2TAG
Plasmid#158138PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d_GgPCFT
Plasmid#165414Purposeexpression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.DepositorInsertGgPCFT (SLC46A1 Chicken, Synthetic)
UseTagsGFP-His8ExpressionYeastMutationPromoterGAL1Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS306Sup35R1
Plasmid#17715DepositorInsertpRS306Sup35R1 (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationAn pRS306 based integrating plasmid designed for …PromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(848).ER
Plasmid#108215PurposeYeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor aDepositorInsertGal4-ERalpha HBD
UseTagsExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH0Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R514G-YFP
Plasmid#29616DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationR514GPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
MS2-ecDHFR
Plasmid#65859PurposeExpresses RNA-binding protein MS2 with eDHFR fusion tagDepositorInsertsUseTagsHA and NLSExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUDR217
Plasmid#113872Purposeexpression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectivelyDepositorInsertsgRNA9-MPH2-3 sgRNA10-MAL11
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWS788
Plasmid#123059Purpose_-factor proteaseDepositorInsertBar1 [3]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mECitrine-kanMX6
Plasmid#105150PurposeYeast gene targetingDepositorInsertmECitrine-kanMX6
UseTagsmECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoterAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MET FLAG103QDProCFPp303
Plasmid#15591DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
HC080
Plasmid#59545PurposeExpresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tagDepositorInsertATG13 (ATG13 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationAvrII site created after start codonPromoterATG13Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXP114
Plasmid#26812DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
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CUP Puf2 YFP p316
Plasmid#15595DepositorInsertPUF2 (PUF2 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDN-NG1Tt
Plasmid#44523DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationPromoterPGal1 (wt)Available sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSP-GM2
Plasmid#64740PurposeYeast expression plasmidDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNX3a-PK3
Plasmid#78346Purposefission yeast epitope taggingDepositorInserts3xPK
kanMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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