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pNB2176

Plasmid

#113395

Purpose

Ty3-IN-GFP; alpha factor inducible

Depositor

Suzanne Sandmeyer

Publication

Beliakova-Bethell et al RNA. 2006 Jan;12(1):94-101. doi: 10.1261

Insert

Ty3 with integrase-green fluorescent protein

Tags

IN-GFP

Expression

Yeast

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZc-Ub-R-HsACTB*-thymosinB-8His

Plasmid

#111149

Purpose

Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Publication

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

N-terminal arginylation

Promoter

AOX1

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMEL15

Plasmid

#107921

Purpose

nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB6677

Plasmid

#106155

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB6638

Plasmid

#106165

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHES834

Plasmid

#87947

Purpose

Expresses mKate2 under control of GAL1 promoter.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mKate2

Use

Synthetic Biology

Expression

Yeast

Promoter

pGal1

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pROS13

Plasmid

#107927

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6-mTFP1-His3MX6

Plasmid

#69850

Purpose

C-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Publication

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Expression

Yeast

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCSJ95

Plasmid

#104343

Purpose

Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Publication

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

FBP1

Tags

3xFLAG

Expression

Yeast

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Publication

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pXNgate21-3HA

Plasmid

#105078

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-TLG1

Plasmid

#105261

Purpose

Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg1 (TLG1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pNXgate32-3HA

Plasmid

#105076

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ183

Plasmid

#98425

Purpose

Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ184

Plasmid

#98426

Purpose

Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-msGFPx3

Plasmid

#105258

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Gga2 portion (GGA2 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-PIK1

Plasmid

#105260

Purpose

Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Pik1 (PIK1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-YPT31

Plasmid

#105263

Purpose

Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt31 (YPT31 )

Tags

iGFP

Expression

Yeast

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pG23A

Plasmid

#102884

Purpose

Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Depositor

Matthias Heinemann

Publication

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTUP1

Plasmid

#104341

Purpose

Express TUP1

Depositor

Alexander Varshavsky

Publication

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

TUP1 (TUP1 Budding Yeast)

Expression

Yeast

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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