8,058 results

1. pICXNH3

   Plasmid

   #49020

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site) and decaHis (…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRW40

   Plasmid

   #130973

   Purpose

   HyPr plasmid with nptII marker

   Depositor

   Chris Todd Hittinger

   Article

   Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

   Insert

   empty backbone
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYC34

   Plasmid

   #63915

   Purpose

   Recipient overexpressing vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 (Saccharomyces cerevisiae)

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG426-GAL-TAF15

   Plasmid

   #181713

   Purpose

   Galactose inducible expression of TAF15

   Depositor

   Alejandro Chavez

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG416-GAL-hnRNPA1

   Plasmid

   #181710

   Purpose

   Galactose inducible expression of hnRNPA1

   Depositor

   Alejandro Chavez

   Insert

   hnRNPA1 (HNRNPA1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS1885

   Plasmid

   #177341

   Purpose

   Expresses Aequorin in Candida albicans using the dominant marker SAT1

   Depositor

   Dominique Sanglard

   Article

   Sanglard et al J Fungi (Basel). 2021 Apr 20;7(4). pii:

   Insert

   Aequorin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ACT1

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFTK020

   Plasmid

   #171292

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   PalcA syn_NoCrea (ANIA_08979) promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCggRO-reporter

   Plasmid

   #124582

   Purpose

   Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

   Depositor

   Matthias Heinemann

   Article

   Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

   Insert

   CggR promoter element
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTAJAK-71 (pESC-NatMXsyn-USER)

   Plasmid

   #78232

   Purpose

   Empty vector, for the insertion of gRNA expression cassettes into the vector.

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJY3919

    Plasmid

    #113332

    Purpose

    Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB4778

    Plasmid

    #106125

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yomKeima-CaURA3

    Plasmid

    #44885

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pPEE47

    Plasmid

    #139426

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPEE46

    Plasmid

    #139425

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p426*-LbSgH

    Plasmid

    #136992

    Purpose

    Helper plasmid for LbCpf1 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINER5

    Plasmid

    #185895

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDDGFP2_leu2d_NRT1.1

    Plasmid

    #58332

    Purpose

    Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

    Insert

    NRT1.1 (NRT1.1 Mustard Weed)
    

    Use

    Tags

    GFP-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. YEp195-CUP1-His-Smt3

    Plasmid

    #99538

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV0753

    Plasmid

    #133487

    Purpose

    Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge