8,058 results

1. pDDGFP-Leu2d-ScVrg4

   Plasmid

   #102334

   Purpose

   Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

   Insert

   YGL225W (VRG4 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. NM R2E2 GFP

   Plasmid

   #1208

   Depositor

   Susan Lindquist

   Article

   Liu et al Nature 1999 Aug 5;400(6744):573-6.

   Insert

   sup35 NM domain (SUP35 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   2 additional copies of second oligopeptide repeat…

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV10.KN

   Plasmid

   #63209

   Purpose

   pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LHP398 - Ub-K48R/K63R

   Plasmid

   #32167

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K48R and K63R

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pGAL-flp(H305L)-NAT

   Plasmid

   #160380

   Purpose

   overexpression of Flp recombinase step-arrest mutant in yeast

   Depositor

   John McCusker

   Insert

   FLP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L

   Promoter

   GAL

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS240

   Plasmid

   #34966

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K533∆, high-affinity epitope

   Promoter

   GAL1

   Available since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNXgate32-3HA

   Plasmid

   #105076

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   3xHA and NubG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH

   Plasmid

   #64178

   Purpose

   harbours marker gene HIS3

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pABY-c25

    Plasmid

    #201769

    Purpose

    Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pI1-MTU-DO-HIS

    Plasmid

    #160142

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pI4-MTU-DO-HPH

    Plasmid

    #160216

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTL-DHDDS

    Plasmid

    #203177

    Purpose

    Centromeric yeast expression vector for hDHDDS

    Depositor

    William Sessa

    Article

    Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

    Insert

    DHDDS (DHDDS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RER2

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Yiplac211 proGAL1-SZ1-RGG-GFP-RGG-RGG

    Plasmid

    #176396

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal SynZip1 coiled coil (Sz1). Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    GAL1 promoter, SZ1-RGG-GFP-RGG-RGG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p415TEF cyto roGFP(iL)-Grx1(human)

    Plasmid

    #65002

    Purpose

    Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    roGFP(iL)

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1234

    Plasmid

    #62367

    Purpose

    pRS305 pPGK1-BirA::LEU2

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA (birA )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXP741

    Plasmid

    #46059

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-GBP-hphMX6

    Plasmid

    #105159

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

    Insert

    GBP-hphMX6
    

    Use

    Tags

    GBP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. EZ-L439

    Plasmid

    #169146

    Purpose

    Encodes OptoINVRT7 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV0327

    Plasmid

    #133488

    Purpose

    Expression of nucleo-cytoplasmic sfGFP

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge