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pENTR221-ITGB1(no stop)

Plasmid

#215453

Purpose

Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway Cloning

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available since

Sept. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-ABL2(WT)

Plasmid

#215469

Purpose

Dox-inducible lentiviral transfer vector with Arg wild-type (WT)

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Lentiviral

Promoter

mCMV, Doxycycline-inducible

Available since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA_mIGg2a_Anti-DYKDDDDK [M2] v2

Plasmid

#236293

Purpose

Mammalian vector expressing Anti-DYKDDDDK [M2] variable region fused to mouse IgG2a heavy chain; to be used with pcDNA_mK-Anti_DYKDDDDK [M2] v2 light chain (Plasmid 236294) to make the antibody.

Depositor

Melina Fan, Addgene Research Program

Insert

Anti-DYKDDDDK [M2] heavy chain variable region fused to mouse IgG2a heavy chain

Tags

Mouse IgG2a Fc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA_mK-Anti_DYKDDDDK [M2] v2

Plasmid

#236294

Purpose

Mammalian vector expressing Anti-DYKDDDDK [M2] variable region fused to mouse Kappa light chain; to be used with pcDNA_mIGg2a_Anti-DYKDDDDK [M2] v2 heavy chain (Plasmid 236293) to make the antibody.

Depositor

Melina Fan, Addgene Research Program

Insert

Anti-DYKDDDDK [M2] light chain variable region fused to mouse kappa light chain

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTIPI_mIGg2a_Anti-DYKDDDDK [M2]

Plasmid

#210654

Purpose

Mammalian expression of Anti-DYKDDDDK [M2] mouse IgG2a heavy chain; to be used with pTIPI_mK-Anti_DYKDDDDK [M2] light chain (Plasmid 210655) to make the antibody.

Depositor

Melina Fan, Addgene Research Program

Insert

Anti-DYKDDDDK [M2] mouse IgG2a heavy chain

Use

Affinity Reagent/ Antibody

Tags

Mouse IgG2a heavy chain

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTIPI_mK-Anti_DYKDDDDK [M2]

Plasmid

#210655

Purpose

Mammalian expression of Anti-DYKDDDDK [M2] kappa light chain; to be used with pTIPI_mIGg2a_Anti-DYKDDDDK [M2] heavy chain (Plasmid 210654) to make the antibody.

Depositor

Melina Fan, Addgene Research Program

Insert

Anti-DYKDDDDK [M2] kappa light chain

Use

Affinity Reagent/ Antibody

Tags

Mouse kappa light chain

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_Ckm_F9

Plasmid

#226140

Purpose

AAV construct for HITI insert with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Publication

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)
EGFP

Use

AAV and CRISPR

Expression

Mammalian

Available since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-HSP90AB1 Fusion Vector

Plasmid

#237196

Purpose

Express NanoLuc(R)-HSP90AB1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

HSP90AB1 (HSP90AB1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-DGKg Fusion Vector

Plasmid

#237186

Purpose

Express NanoLuc(R)-DGKG Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

DGKG (DGKG Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-CA2 Fusion Vector

Plasmid

#237187

Purpose

Express NanoLuc(R)-CA2 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

CA2 (CA2 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-IRAK2 Fusion Vector

Plasmid

#237181

Purpose

Express NanoLuc(R)-IRAK2 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

IRAK2 (IRAK2 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoLuc-TOM7 Fusion Vector

Plasmid

#237166

Purpose

Express NanoLuc(R)-TOM7 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

TOM7 (TOMM7 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET Assay Vector, CSNK1A1-NanoLuc

Plasmid

#237143

Purpose

Express CSNK1A1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

CSNK1A1 (CSNK1A1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET Assay Vector, NanoLuc-MLL1

Plasmid

#237135

Purpose

Express NanoLuc(R)-MLL1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

MLL1 (KMT2A Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET MLL1/WDR5 Assay Acceptor Vector, WDR5

Plasmid

#237140

Purpose

Express WDR5-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

WDR5 (WDR5 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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LgBiT-NRF2 Fusion Vector

Plasmid

#237127

Purpose

Express LgBiT-NRF2 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

NRF2 (NFE2L2 Human)

Tags

LgBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PD-L1-NanoLuc Fusion Vector

Plasmid

#237129

Purpose

Express PD-L1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

PD-L1 (CD274 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NanoBRET Assay Vector, EED-NanoLuc

Plasmid

#237130

Purpose

Express EED-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

EED (EED Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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LgBiT-CALM1 BiBiT-Ready Vector

Plasmid

#237119

Purpose

Express LgBiT-CALM1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

CALM1 (CALM1 Human)

Tags

LgBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SmBiT-MAFG Fusion Vector

Plasmid

#237122

Purpose

Express SmBiT-MAFG Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has service

20 µg DNA, 50 µg Transfection-Ready DNA, and 100 µg Transfection-Ready DNA

Insert

MAFG (MAFG Human)

Tags

SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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