1,892 results

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EFF-1::V5

Plasmid

#132961

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

EFF-1::V5

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMA122 - peel-1 negative selection

Plasmid

#34873

Purpose

Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

peel-1 (peel-1 Nematode)

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ601 - Peft-3 Mos1 transposase

Plasmid

#34874

Purpose

Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Mos1 transpoase

Use

Worm targeting

Promoter

eef-1A.1 (eft-3)

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ356 - MCS(cxTi10816, IV)

Plasmid

#34871

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pET_RGG-GFP-RGG

Plasmid

#124948

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP and His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-sCAG-GluClβ.CreON

Plasmid

#196996

Purpose

Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

Depositor

David Bennett

Article

Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

Insert

GluClβ -YFP

Use

AAV and Cre/Lox

Tags

YFP

Promoter

short CAG

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG_withTEVsite

Plasmid

#124940

Purpose

Expression of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

with TEV site between RGG domains

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-sCAG-GluClα.CreON

Plasmid

#196995

Purpose

Cre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.

Depositor

David Bennett

Article

Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

Insert

GluClα -Cerulean

Use

AAV and Cre/Lox

Tags

Cerulean

Promoter

short CAG

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSM.wrmScarlet1-10::SL2::GFP1-10

Plasmid

#234318

Purpose

For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

Insert

wrmScarlet1-10::SL2::GFP1-10

Expression

Worm

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSKI

Plasmid

#232484

Purpose

pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

Depositor

Carlos Silva García

Article

Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

Insert

pSKI

Expression

Worm

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJH3557

Plasmid

#191444

Purpose

Pttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Tags

wCherry

Expression

Worm

Promoter

Pttr-39

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSA102

Plasmid

#59788

Purpose

drives mCherry in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
mCherry (worm)
unc-54 3' UTR

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Promoter

myo-2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDD346

Plasmid

#133311

Purpose

SapTrap mNG-C1 donor

Depositor

Daniel Dickinson

Insert

C. elegans codon-optimized mNeonGreen

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans codon-optimized mNeonGreen

Expression

Worm

Promoter

None

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ782 - HygroR

Plasmid

#190933

Purpose

Hygromycin resistance co-injection marker

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Hygromycin resistance gene

Expression

Worm

Promoter

rps-0

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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