Addgene

We narrowed to 30 results for: waugh

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pDZ2087

Plasmid

#92414

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

Insert

TEV protease

Use

Tags

MBP (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

tac

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Use

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Use

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pSRK2706

Plasmid

#78571

Purpose

Expresses untagged human rhinovirus 3C protease in E. coli

Depositor

David Waugh

Article

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

human rhinovirus 3C protease (HRVBgp1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-HisMBP

Plasmid

#11085

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Article

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Use

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Mutation

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pDW363

Plasmid

#8842

Depositor

David Waugh

Article

Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Type

Empty backbone

Use

Tags

MBP and biotin acceptor peptide

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Use

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pRK603

Plasmid

#8831

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease, S219D mutant

Use

Tags

Expression

Bacterial

Mutation

S219D mutant

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK792

Plasmid

#8830

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219P mutant

Use

Tags

His, MBP (cleaved), and polyarginine

Expression

Bacterial

Mutation

S219P mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pSRK2721

Plasmid

#78572

Purpose

Gateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions

Depositor

David Waugh

Article

Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

Insert

Gateway cloning cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRK1035

Plasmid

#8832

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Use

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pKM586

Plasmid

#19978

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease (TEVgp1 tobacco etch virus)

Use

Tags

Expression

Bacterial

Mutation

S219D mutant

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK1037

Plasmid

#8833

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease, catalytic domain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pKM596

Plasmid

#8837

Depositor

David Waugh

Article

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Use

Tags

E. coli MBP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pKM631

Plasmid

#17727

Depositor

David Waugh

Article

Kapust et al Biochem Biophys Res Commun. 2002 Jun 28.

Insert

NusG (nusG Aquifex aeolicus)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pBA2257

Plasmid

#38151

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

MBP-proMeCPA fusion protein

Use

Tags

maltose binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pKM839

Plasmid

#8838

Depositor

David Waugh

Article

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Use

Tags

P. furiosus MBP

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pDW464

Plasmid

#8845

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Use

Tags

biotin acceptor peptide

Expression

Insect

Mutation

Promoter

Available since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

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pDW465

Plasmid

#8846

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Use

Tags

biotin acceptor peptide

Expression

Insect

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK1036

Plasmid

#17728

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

NusG (nusG Aquifex aeolicus)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pHPK1212

Plasmid

#8834

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

tobacco vein mottling virus protease, catalytic domain

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDW446

Plasmid

#8844

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Use

Tags

biotin acceptor peptide

Expression

Insect

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p1177-X1-8

Plasmid

#38150

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2011 May;77(1):53-61

Insert

Carboxypeptidase A

Use

Tags

HHHHHHRR

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pDW445

Plasmid

#8843

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Use

Tags

biotin acceptor peptide

Expression

Insect

Mutation

Promoter

Available since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM1000

Plasmid

#8840

Depositor

David Waugh

Article

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Use

Tags

T. maritima MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pJF1106

Plasmid

#8836

Depositor

David Waugh

Article

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Use

Tags

Yersinia pestis MBP

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM1137

Plasmid

#8841

Depositor

David Waugh

Article

Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

Type

Empty backbone

Use

Tags

V. cholera MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Use

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
HisMBP CFlm25

Plasmid

#78458

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie

Article

Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

Insert

Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only