1. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pX330-sgRNA_Dicer_2

   Plasmid

   #68808

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX330-sgRNA_Dicer_3

   Plasmid

   #68809

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-sgRNA_Dicer_1

   Plasmid

   #68807

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330_EZH2gRNA15

   Plasmid

   #199587

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330_EZH2gRNA16

   Plasmid

   #199588

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330_EZH2gRNA17

   Plasmid

   #199589

   Purpose

   Contains guide RNA to 3' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330_EZH2gRNA14

   Plasmid

   #199586

   Purpose

   Contains guide RNA to 5' end of mouse EZH2 gene

   Depositor

   Mauro Calabrese

   Article

   Cherney et al bioRxiv. 2023 Apr 3:2023.04.03.535391. d

   Insert

   EZH2 (Ezh2 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330_gRNA_IG-DMR-2

   Plasmid

   #159932

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330_gRNA_H19-DMR-1

    Plasmid

    #159929

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRNF219.1.0-gDNA

    Plasmid

    #132464

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RNF219
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330_SAFBgRNA9

    Plasmid

    #196108

    Purpose

    Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBACH2.2.0-gDNA

    Plasmid

    #176375

    Purpose

    Expresses spCas9 and gRNA sequence targeting BACH2

    Depositor

    Barbara Stranger, Kevin White

    Insert

    BACH2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSOX2.1.0-gDNA

    Plasmid

    #176382

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX2

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSOX11.1.0-gDNA

    Plasmid

    #176378

    Purpose

    Expresses spCas9 and gRNA sequence targeting SOX11

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX11 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRERF1.1.0-gDNA

    Plasmid

    #176384

    Purpose

    Expresses spCas9 and gRNA sequence targeting TRERF1

    Depositor

    Barbara Stranger, Kevin White

    Insert

    TRERF1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZNF627.1.0-gDNA

    Plasmid

    #176383

    Purpose

    Expresses spCas9 and gRNA sequence targeting ZNF627

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZNF627 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOSR1.1.0-gDNA

    Plasmid

    #176381

    Purpose

    Expresses spCas9 and gRNA sequence targeting OSR1

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OSR1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZNF608.2.0-gDNA

    Plasmid

    #176380

    Purpose

    Expresses spCas9 and gRNA sequence targeting ZNF608

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZNF608 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPOU6F1.1.0-gDNA

    Plasmid

    #176377

    Purpose

    Expresses spCas9 and gRNA sequence targeting POU6F1

    Depositor

    Barbara Stranger, Kevin White

    Insert

    POU6F1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pZC3H10.1.0-gDNA

    Plasmid

    #176385

    Purpose

    Expresses spCas9 and gRNA sequence targeting ZC3H10

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZC3H10 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZIC3.1.0-gDNA

    Plasmid

    #176379

    Purpose

    Expresses spCas9 and gRNA sequence targeting ZIC3

    Depositor

    Barbara Stranger, Kevin White

    Insert

    ZIC3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOTX2.1.0-gDNA

    Plasmid

    #176376

    Purpose

    Expresses spCas9 and gRNA sequence targeting OTX2

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OTX2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNR2E3.1.0-gDNA

    Plasmid

    #176386

    Purpose

    Expresses spCas9 and gRNA sequence targeting NR2E3

    Depositor

    Barbara Stranger, Kevin White

    Insert

    NR2E3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPHB.1.0-gDNA

    Plasmid

    #132453

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    PHB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZNF668.1.0-gDNA

    Plasmid

    #132463

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF668 (ZNF668 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCENPBD1.1.0-gDNA

    Plasmid

    #132460

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CENPBD1 (CENPBD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTHAP7.1.0-gDNA

    Plasmid

    #132439

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    THAP7 (THAP7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTOX.1.0-gDNA

    Plasmid

    #132470

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TOX (TOX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pZNF132.1.0-gDNA

    Plasmid

    #132468

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF132 (ZNF132 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSOX13.1.0-gDNA

    Plasmid

    #132466

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    SOX13 (SOX13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPHTF2.1.0-gDNA

    Plasmid

    #132465

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    PHTF2 (PHTF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRHOXF2B.1.0-gDNA

    Plasmid

    #132462

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RHOXF2B (RHOXF2B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRFX7.1.0-gDNA

    Plasmid

    #132455

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RFX7 (RFX7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZNF830.1.0-gDNA

    Plasmid

    #132450

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF830 (ZNF830 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pZNF609.1.0-gDNA

    Plasmid

    #132447

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF609 (ZNF609 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pZNF780A.1.0-gDNA

    Plasmid

    #132446

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF780A (ZNF780A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pZNF518B.1.0-gDNA

    Plasmid

    #132442

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF518B (ZNF518B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pZNF551.1.0-gDNA

    Plasmid

    #132441

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF551 (ZNF551 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pZNF319.1.0-gDNA

    Plasmid

    #132438

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF319 (ZNF319 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPATZ1.1.0-gDNA

    Plasmid

    #132476

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    PATZ1 (PATZ1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pZNF449-R.1.0-gDNA

    Plasmid

    #132469

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF449 (ZNF449 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pZNF302.1.0-gDNA

    Plasmid

    #132467

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF302 (ZNF302 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pZNF253.1.0-gDNA

    Plasmid

    #132461

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF253 (ZNF253 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pZFPM2.1.0-gDNA

    Plasmid

    #132459

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZFPM2 (ZFPM2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pZNF3.1.0-gDNA

    Plasmid

    #132440

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF3 (ZNF3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pTSHZ1.1.0-gDNA

    Plasmid

    #132434

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TSHZ1 (TSHZ1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRARB.1.0-gDNA

    Plasmid

    #132471

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RARB (RARB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge