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Showing: 1 - 50 of 12902 results

  1. dTAG-IRF8

    Plasmid
    #190620
    Purpose
    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    IRF8 (IRF8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance for sgIR…
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    190620-plasmid-map-sequence-id-372992

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  5. pSL21-VEX

    Plasmid
    #158230
    Purpose
    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)
    Depositor
    Junwei Shi
    Article
    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter for crRNA expression and EFS promoter…
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158230-plasmid-map-sequence-id-317503

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  13. ZMYND8-dTAG

    Plasmid
    #190617
    Purpose
    Lentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    ZMYND8 (ZMYND8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V and Flag, 2xHA
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance at aa214…
    Promoter
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190617-plasmid-map-sequence-id-373000

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  15. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid
    #221385
    Purpose
    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene
    Depositor
    Junwei Shi
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    221385-plasmid-map-sequence-id-435944

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  18. Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228373
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Asparagine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 (SHI02794); determination of S. himalayana AsnRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana AsnRS evm.model.fragScaff scaffold 419.110 (SHI02794)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228373-plasmid-map-sequence-id-456195

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  19. Shim_CytC_87.18_SHI04112_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228375
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Cytochrome C with demonstrated mito targeting; proof-of-concept for Plasmid 228371
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana Cytochrome C with demonstrated mito targeting.
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228375-plasmid-map-sequence-id-455854

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  20. Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228377
    Purpose
    Expresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana GlyRS evm.model.fragScaff scaffold 87.19 (SHI04113RA)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228377-plasmid-map-sequence-id-455855

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  21. Shim_ProRS_3278.3.7_SHI28108_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228379
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Proline aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA); determination of S. himalayana ProRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana ProRS evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228379-plasmid-map-sequence-id-455871

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  22. Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228381
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228381-plasmid-map-sequence-id-455873

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  24. Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228374
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228374-plasmid-map-sequence-id-455849

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  25. Shim_GluRS_87.31.3_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228376
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Glutamate aminoacyl-tRNA synthetase evm.model.fragScaff_scaffold_87.31.3; determination of S. himalayana GluRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana GluRS evm.model.fragScaff_scaffold_87.31.3
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228376-plasmid-map-sequence-id-454891

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  26. Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228378
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Methionine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; determination of S. himalayana MetRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana MetRS evm.model.fragScaff scaffold 440.10
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228378-plasmid-map-sequence-id-455870

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  27. Shim_ThrRS_3895.3_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid
    #228380
    Purpose
    Expresses N-term transit peptide from Sapria himalayana Threonine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3895.3; determination of S. himalayana ThrRS target localization
    Depositor
    Daniel Sloan
    Article
    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    N-terminal transit peptide from S. himalayana ThrRS evm.model.fragScaff scaffold 3895.3
    Use
    Tags
    Green Florescent Protien
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228380-plasmid-map-sequence-id-455872

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  29. SHIP2-C1

    Plasmid
    #214905
    Purpose
    expression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motif
    Depositor
    Pavel Krejčí
    Article
    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608
    Insert
    human SHIP2 aa 740-1258 (INPPL1 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    delta 1-739aa
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214905-plasmid-map-sequence-id-426097

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  40. StarShip01

    Plasmid
    #164505
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164505-plasmid-map-sequence-id-324722

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  41. StarShip02

    Plasmid
    #164506
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164506-plasmid-map-sequence-id-324723

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  42. StarShip03

    Plasmid
    #164507
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    164507-plasmid-map-sequence-id-324724

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  43. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  49. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid
    #200408
    Purpose
    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    iRFP720 (frameshift mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200408-plasmid-map-sequence-id-396809

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Showing: 1 - 50 of 12902 results