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Showing: 1 - 50 of 153 results

  1. pCW57.1_SPTLC1_WT

    Plasmid
    #181920
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181920-plasmid-map-sequence-id-361674

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  3. PLVX_SPTA1_IGF2BP1_IRES_PURO

    Plasmid
    #100016
    Purpose
    To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.
    Depositor
    Jeffery Miller
    Article
    de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11
    Insert
    Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SPTA1 erythroid specific promoter
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100016-plasmid-map-sequence-id-194507

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  4. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Tags
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

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  11. pRS415-SPT6

    Plasmid
    #10993
    Depositor
    Francisco Malagon
    Insert
    SPT6 (SPT6 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    10993-plasmid-map-sequence-id-6828

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  19. pT7-V5-SBP-C1-HsPTB1

    Plasmid
    #64925
    Purpose
    Expresses V5- and SBP-tagged PTB1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    PTB1 (PTBP1 Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64925-plasmid-map-sequence-id-109671

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  20. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Use
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

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  27. pcDNA3.1-MS2-HA-HsPTB2_AA

    Plasmid
    #148238
    Purpose
    Mammalian Expression of HsPTB2
    Depositor
    Elisa Izaurralde
    Insert
    HsPTB2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148238-plasmid-map-sequence-id-290686

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  28. pcDNA3.1-MS2-HA-HsPTB1_Z

    Plasmid
    #148129
    Purpose
    Mammalian Expression of HsPTB1
    Depositor
    Elisa Izaurralde
    Insert
    HsPTB1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    4 silent mutations compared to transcript variant…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148129-plasmid-map-sequence-id-290577

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  29. pT7-V5-SBP-C1-HsPTB2_AA

    Plasmid
    #148253
    Purpose
    Mammalian Expression of HsPTB2
    Depositor
    Elisa Izaurralde
    Insert
    HsPTB2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148253-plasmid-map-sequence-id-290701

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  34. Spt2-K166R-GFP

    Plasmid
    #115573
    Purpose
    Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    SPT2
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    Lysine 166 mutated to Arginine
    Promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  35. pH6-TEV-spTorA-GFP(C)

    Plasmid
    #169041
    Purpose
    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    H6-spTorA-GFP
    Use
    Tags
    6xHis, TEV cleavage site, and spTorA (signal pept…
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169041-plasmid-map-sequence-id-346121

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  39. pENTR E2 HSPter-RPS5Ap-mito

    Plasmid
    #163146
    Purpose
    Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF
    Depositor
    Shin-ichi Arimura
    Article
    Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1
    Insert
    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163146-plasmid-map-sequence-id-320768

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  47. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid
    #227084
    Purpose
    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    STREPii-FLAG and SV40 NLS 1
    Expression
    Yeast
    Mutation
    Promoter
    Gal1-10
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227084-plasmid-map-sequence-id-451056

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  50. pKTop::sptse5-CT (1169-1229)

    Plasmid
    #192948
    Purpose
    For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1229)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1229)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192948-plasmid-map-sequence-id-380616

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Showing: 1 - 50 of 153 results