Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 327 results for: IPTG

Clear keywords
Showing: 1 - 20 of 327 results

  1. pET15bHis6-pG-MNase

    Plasmid
    #124886
    Purpose
    To transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.
    Depositor
    Keji Zhao
    Insert
    protein G-MNase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124886-plasmid-map-sequence-id-243685

    Enlarge

  2. pEF1-Pls4-sfGFP

    Plasmid
    #205751
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls4 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205751-plasmid-map-sequence-id-478437

    Enlarge

  3. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

    Enlarge

  4. pEF1-Pls1-sfGFP

    Plasmid
    #205748
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls1 (Phyperspank mutant, IPTG inducible)
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205748-plasmid-map-sequence-id-483889

    Enlarge

  5. pEF1-Pls2-sfGFP

    Plasmid
    #205749
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls2 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/low strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls2 (Phyperspank mutant, IPTG inducible)
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205749-plasmid-map-sequence-id-483890

    Enlarge

  6. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Expression
    Bacterial
    Promoter
    IPTG
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167546-plasmid-map-sequence-id-332204

    Enlarge

  8. pTALE64

    Plasmid
    #35189
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Transcription Factor
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35189-plasmid-map-sequence-id-68899

    Enlarge

  9. pTALE64G

    Plasmid
    #35188
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition
    Depositor
    Gang Bao
    Insert
    TAL Effector-VP64-T2A-eGFP
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain, T2A-GFP
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35188-plasmid-map-sequence-id-42839

    Enlarge

  10. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40786-plasmid-map-sequence-id-68901

    Enlarge

  11. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40787-plasmid-map-sequence-id-68902

    Enlarge

  12. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40788-plasmid-map-sequence-id-68904

    Enlarge

  13. TALEN_C18

    Plasmid
    #47974
    Purpose
    Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    Shortened C-Terminus,Codon Optimized FokI Endonuc…
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47974-plasmid-map-sequence-id-68905

    Enlarge

  16. AWP-029

    Plasmid
    #213966
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Segatella bryantii, Synthetic)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213966-plasmid-map-sequence-id-429164

    Enlarge

  17. AWP-031

    Plasmid
    #213967
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Segatella bryantii, Synthetic)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213967-plasmid-map-sequence-id-429166

    Enlarge

  18. STK034_pSTK-0A-Phyperspank

    Plasmid
    #235371
    Purpose
    Medium constitutive promoter. Note: Requires LacI to be inducible with IPTG.
    Depositor
    Tom Ellis
    Insert
    Phyperspank
    Expression
    Bacterial
    Mutation
    No
    Available Since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235371-plasmid-map-sequence-id-502630

    Enlarge

  19. P2072

    Plasmid
    #248832
    Purpose
    Lac12 for IPTG import
    Depositor
    Joerg Stelling
    Insert
    Lac12
    Expression
    Yeast
    Promoter
    pACT1
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248832-plasmid-map-sequence-id-495622

    Enlarge

  20. P2882

    Plasmid
    #248833
    Purpose
    Lac12 for IPTG import
    Depositor
    Joerg Stelling
    Insert
    Lac12
    Expression
    Yeast
    Promoter
    pACT1
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248833-plasmid-map-sequence-id-495623

    Enlarge

Showing: 1 - 20 of 327 results