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pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Use

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 DSred express2

Plasmid

#128040

Purpose

Mammalian expression vector with N terminal DsRed express2

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

DsRed Express2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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6HisVDAC1/pCDNA3

Plasmid

#197320

Purpose

Plasmid expressing an antigen targeting the VDAC1 transporter

Depositor

Dan Minor

Insert

VDAC1 (VDAC1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Shank2-HA /pCDNA3

Plasmid

#197334

Purpose

Plasmid expressing an antigen targeting Shank2

Depositor

Morgan Sheng

Insert

Shank2 (Shank2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-C-Luc

Plasmid

#185358

Purpose

For mammalian expression of C-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 398-550).

Depositor

Ayaz Najafov

Insert

Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Luc

Plasmid

#185357

Purpose

For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).

Depositor

Ayaz Najafov

Insert

Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA

Plasmid

#128034

Purpose

Mammalian expression vector with N terminal HA tag

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_RapACR_T111C_N248Q-EYFP

Plasmid

#119197

Purpose

Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q

Depositor

John Spudich

Insert

Anion channelrhodopsin RapACR fast mutant T111C_N248Q

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed threonine 111 to cysteine and asparagine …

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-m-Flt4

Plasmid

#119232

Purpose

Plasmid encodes full-length murine VEGFR-3 gene cloned in mammalian expression vector.

Depositor

Kari Alitalo

Insert

Vegfr3 (Flt4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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HCN3/pCDNA3.1

Plasmid

#197293

Purpose

Plasmid expressing an antigen targeting the HCN3 ion channel subunit

Depositor

Dane Chetkovich

Insert

HCN3 (Hcn3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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SynCAM4/pCDNA3

Plasmid

#197328

Purpose

Plasmid expressing an antigen targeting NECL4/SynCAM4

Depositor

Matthew Rasband

Insert

SynCAM4 (Cadm4 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-(-)-Pur

Plasmid

#200458

Purpose

Eukaryotic expression vector

Depositor

Bertrand Collet

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-3-VEGFR3

Plasmid

#119230

Purpose

Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.

Depositor

Kari Alitalo

Insert

VEGFR3 (FLT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_EG.5.1

Plasmid

#207641

Purpose

pseudovirus plasmid that expresses spike of EG.5.1

Depositor

David Nemazee

Insert

Spike (EG.5.1) (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

Q52H, F456L on the basis of XBB.1.5

Promoter

CMV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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Rat MMP-9 pcDNA3.1

Plasmid

#196951

Purpose

Plasmid expressing an antigen targeting the MMP9 metalloproteinase

Depositor

James Trimmer

Insert

mmp-9 (Mmp9 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ccdB-V5

Plasmid

#214985

Purpose

Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag

Depositor

Wade Harper

Type

Empty backbone

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 acGFP

Plasmid

#128047

Purpose

Mammalian expression vector with N terminal AcGFP

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Mfn1

Plasmid

#191955

Purpose

Expression of Mfn1, a mediator of mitochondrial fusion.

Depositor

Luca Scorrano

Insert

Mfn1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Mfn2

Plasmid

#191960

Purpose

Expression of the mitochondrial GTPase Mfn2.

Depositor

Luca Scorrano

Insert

Mfn2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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SEGFP/pcDNA3

Plasmid

#51968

Purpose

mammalian expression of SEGFP, a super enhanced green fluorescent protein

Depositor

Takeharu Nagai

Insert

SEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only