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Plasmid#215407PurposeExpression construct for HotPETase gene, codon optimized for expression in E. coliDepositorInsertHotPETase
UseTagsExpressionBacterialMutationS121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…PromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
UseTags6xHis TagExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b FAST-PETase
Plasmid#225760PurposeExpresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tagDepositorInsertFAST-PETase
UseTags6xHis TagExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b Hot-PETase
Plasmid#225761PurposeExpresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tagDepositorInsertHot-PETase
UseTags6xHis TagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase-W159H-S238F
Plasmid#112203PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationW159H, S238FPromoterN/AAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21b(+)-Is-PETase-W185A
Plasmid#112204PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationW185APromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(40nm)-LPETGG
Plasmid#90475PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(40nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(120nm)-LPETGG
Plasmid#90472PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(120nm)-LPETGG
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-LPETGG
Plasmid#91697PurposeCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGGDepositorInsertCohIII-CBM-HIS-LPETGG
UseTagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40731DepositorInsertAurora B FRET-sensor
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40571DepositorInsertAurora B FRET-sensor N-terminally fused to histone 2B
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-BiFC
Plasmid#87856PurposepETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.DepositorInsertsEncodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus
UseTagsBiFC C-terminal fragmentExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-mKelly2
Plasmid#119783PurposeExpresses mKelly2 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly2
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-PfuX7
Plasmid#182364PurposeExpresses PfuX7 DNA polymeraseDepositorInsertPfuX7 DNA polymerase
UseTagsHis-tagExpressionBacterialMutationV93QPromoterT7Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneUseTagsHisX6ExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
UseTags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp
Plasmid#159617PurposeExpression plasmid for Sfp phosphopantetheinyl transferaseDepositorInsertSfp
UseTags6HISExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -