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Plasmid#48627PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.DepositorInsertSV40 early polyadenylation signal cassette
UseGateway entry vectorTagsExpressionMutationPromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
mpCHECK2
Plasmid#167576PurposeThe mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
p(A)n-PAUSE
Plasmid#118068PurposeTranscription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin geneDepositorInsertSynthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
HB901: pMVP (L3-L2) polyA
Plasmid#121746PurposepMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertPolyadenylation signal (polyA)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2
Plasmid#91802PurposeExpresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be usedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-Cre
Plasmid#40591PurposeMouse astrocyte expression of CreDepositorInsertCre recombinase (GFAP Human, Mouse, Phage)
UseCre/Lox and Mouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionMammalianMutationConstruct contains a nuclear-targeted Cre recombi…PromoterhGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP_CD47_LU
Plasmid#65474PurposeGFP tagged CD47 with its own long 3'UTRDepositorInsertsSignal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
251 pTol2Dest
Plasmid#73484Purposepromoterless Tol2 vector with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsertattR1/attR2 cassette
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
252 pTol2-MTDest
Plasmid#73485Purposepromoterless Tol2 vector with 6xmyc tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsert6xmyc/attR1/attR2
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
366 pTol2-CherryDest
Plasmid#73487Purposepromoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signalDepositorInsertmCherry-attR1/attR2
UseTagsExpressionMutationH30R (please see depositor comments below)PromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe1889 HC_Kan_SV40-polyA_p11
Plasmid#100678Purposepart designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertSV40-polyA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH71431
Plasmid#50344DepositorInsert3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH41421
Plasmid#50339DepositorInsert3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH41414
Plasmid#50337DepositorInsert3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH41432
Plasmid#50343DepositorInsert3'UTR, polyadenylation signal/terminator, ocs (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH72400
Plasmid#50338DepositorInsert3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
YCe1901 HC_Kan_PGKpA_p22
Plasmid#100683Purposepart designed to occupy position 22 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertPGKpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH77901
Plasmid#50341DepositorInsert3'UTR, polyadenylation signal/terminator, mas (A. tumefaciens)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
YCe2030 HC_Kan_bGHpA_p16
Plasmid#100688Purposepart designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertbGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only