2,293 results

1. Shank3-HA/pRK5

   Plasmid

   #197335

   Purpose

   Plasmid expressing an antigen targeting Shank3

   Depositor

   Morgan Sheng

   Insert

   Shank3 (Shank3 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

   Plasmid

   #118273

   Purpose

   pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mApple-LAMP1-phLuorin-N-8

   Plasmid

   #54918

   Purpose

   Localization: Lysosome Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   LAMP1 phLuorin
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Lysosomes-20 double labeled with phLuorin

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSyn.iGluSnFR3.v857.SGZ

   Plasmid

   #178330

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. alpha-0 CFP in pcDNA3.1

   Plasmid

   #36396

   Depositor

   Narasimhan Gautam

   Article

   Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

   Insert

   G protein alpha 0 (Gnao1 Rat)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted into the KpnI-SpeI sites engineered …

   Promoter

   cmv

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

7. CMV mRuby3-Synapsin1a

   Plasmid

   #187896

   Purpose

   For labeling pre-synaptic terminals

   Depositor

   Timothy Ryan

   Insert

   Synapsin1a
   

   Tags

   mRuby3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-14-3-3 gamma

   Plasmid

   #13274

   Depositor

   Michael Yaffe

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVQ CMV NanoV1-2a-EGFP ferritin

   Plasmid

   #79649

   Purpose

   Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

   Depositor

   Jeffrey Friedman

   Article

   Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

   Inserts

   transient receptor potential vanillin 1 (Trpv1 Rat)
   camelid anti-GFP nanobody
   Enhanced green fluorescent protein
   mouse ferritin light chain (Ftl1 Mouse)
   mouse ferritin heavy chain (Fth1 Mouse)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSyn.iGluSnFR3.v857.GPI

    Plasmid

    #178331

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIneoMyc rat SAPAP1

    Plasmid

    #40215

    Depositor

    Yoshimi Takai, Yutaka Hata

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP1 (Dlgap1 Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Beta-arrestin2 GFP WT

    Plasmid

    #35411

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-ERK2

    Plasmid

    #37145

    Depositor

    Rony Seger

    Article

    Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

    Insert

    ERK2 (MAPK1 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-6 deleted as a result of cloning strategy

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pORANGE GFP-Grin2a KI

    Plasmid

    #131486

    Purpose

    Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2a Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-CMV-Galpha-i3 ONE-GO

    Plasmid

    #189737

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i3 ONE-GO
    GNAI3 (Gnai3 Rat)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. μ2-HA-WT

    Plasmid

    #32752

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

    Plasmid

    #62044

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LAP2beta (Tmpo Rat)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEYFP-NR1a

    Plasmid

    #17928

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid

    #127392

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Aspartic acid and Threon…

    Promoter

    pCAG

    Available Since

    July 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-myc-ERK2-L4A-MEK1_fusion

    Plasmid

    #39197

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L4A = L33A, L37A, L40A, L42A

    Promoter

    CMV

    Available Since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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