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Showing: 361 - 400 of 482 results

  3. MBP-g0s2

    Plasmid
    #179294
    Purpose
    E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    mature maltose binding protein + enterokinase cle…
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    Ptac
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179294-plasmid-map-sequence-id-354917

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  4. pTT5-hSDC1-S15A

    Plasmid
    #52363
    Purpose
    expresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    S15A and R95Q (according to numbering in Fig. 2A …
    Promoter
    CMV
    Available Since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pSUMO-HsSyx3

    Plasmid
    #179288
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179288-plasmid-map-sequence-id-354924

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  8. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

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  9. pTT5-hSDC1-4

    Plasmid
    #52375
    Purpose
    expresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    GAGAL of syndecan-1 replaced with GDLDD of SDC4; …
    Promoter
    CMV
    Available Since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pKP2188

    Plasmid
    #167642
    Purpose
    Express FepA V16C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA V16C, I14P
    Expression
    Bacterial
    Mutation
    FepA with V16C, I14P mutations. (They are numbere…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167642-plasmid-map-sequence-id-337262

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  14. pKP1410

    Plasmid
    #167634
    Purpose
    Express FepA D12C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA D12C
    Expression
    Bacterial
    Mutation
    FepA with D12C mutation. (Numbering based on star…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167634-plasmid-map-sequence-id-337287

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  19. SAGA032

    Plasmid
    #240781
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240781-plasmid-map-sequence-id-484013

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  20. SAGA031

    Plasmid
    #240780
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240780-plasmid-map-sequence-id-484012

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  21. SAGA030

    Plasmid
    #240779
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240779-plasmid-map-sequence-id-484011

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  22. SAGA023

    Plasmid
    #240772
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240772-plasmid-map-sequence-id-484002

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  23. SAGA022

    Plasmid
    #240771
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240771-plasmid-map-sequence-id-483999

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  24. SAGA021

    Plasmid
    #240770
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240770-plasmid-map-sequence-id-483997

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  25. SAGA015

    Plasmid
    #240764
    Purpose
    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15A PrhaBAD BCD_low - gfp - tetAp15A- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240764-plasmid-map-sequence-id-483929

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  26. SAGA014

    Plasmid
    #240763
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 PrhaBAD BCD_high - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240763-plasmid-map-sequence-id-483928

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  27. SAGA013

    Plasmid
    #240762
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 PrhaBAD BCD_medium - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240762-plasmid-map-sequence-id-483927

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  28. SAGA012

    Plasmid
    #240761
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 PrhaBAD BCD_low - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240761-plasmid-map-sequence-id-483926

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  29. SAGA006

    Plasmid
    #240755
    Purpose
    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15A J23100 BCD_high - gfp - tetAp15A- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240755-plasmid-map-sequence-id-483916

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  30. SAGA004

    Plasmid
    #240753
    Purpose
    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15A J23100 BCD_low - gfp - tetAp15A- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240753-plasmid-map-sequence-id-483914

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  31. SAGA003

    Plasmid
    #240752
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 J23100 BCD_high - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240752-plasmid-map-sequence-id-483912

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  32. SAGA002

    Plasmid
    #240751
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 J23100 BCD_medium - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240751-plasmid-map-sequence-id-483911

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  33. SAGA001

    Plasmid
    #240750
    Purpose
    Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 J23100 BCD_low - gfp - tetARK2- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240750-plasmid-map-sequence-id-483909

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  34. SAGA019

    Plasmid
    #240768
    Purpose
    Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA pBR322-ROP PrhaBAD BCD_medium - gfp -tetApBR322-ROP- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240768-plasmid-map-sequence-id-483933

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  35. SAGA018

    Plasmid
    #240767
    Purpose
    Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA pBR322-ROP PrhaBAD BCD_low - gfp - tetApBR322-ROP- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240767-plasmid-map-sequence-id-483932

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  36. SAGA009

    Plasmid
    #240758
    Purpose
    Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA pBR322-ROP J23100 BCD_high - gfp - tetApBR322-ROP- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240758-plasmid-map-sequence-id-483922

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  37. SAGA008

    Plasmid
    #240757
    Purpose
    Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA pBR322-ROP J23100 BCD_medium - gfp -tetApBR322-ROP- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240757-plasmid-map-sequence-id-483921

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  38. SAGA007

    Plasmid
    #240756
    Purpose
    Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA pBR322-ROP J23100 BCD_low - gfp - tetApBR322-ROP- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240756-plasmid-map-sequence-id-483919

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Showing: 361 - 400 of 482 results