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Addgene

We narrowed to 1,992 results for: RP1

Showing: 361 - 400 of 1992 results
  1. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53206
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and TRP1
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

    Plasmid
    #217325
    Purpose
    Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells
    Depositor
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    N61K
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

    Plasmid
    #217324
    Purpose
    Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid
    #217320
    Purpose
    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

    Plasmid
    #217321
    Purpose
    Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

    Plasmid
    #166814
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E1322R
    Promoter
    CMV
    Available since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid
    #166811
    Purpose
    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts
    Depositor
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    LL1193EE, P1214R, K1277E
    Promoter
    CMV
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid
    #166812
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    H1276G
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

    Plasmid
    #166813
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Insert
    NLRP1 (NLRP1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K1277E
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pUASP Flag Rab32 CA

    Plasmid
    #44834
    Depositor
    Insert
    Rab32 (Rab32 Fly)
    Use
    Drosophila
    Tags
    Flag
    Expression
    Insect
    Mutation
    Q79L (constitutive active)
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 361 - 400 of 1992 results