We narrowed to 1,305 results for: pET28a

1. pET28a-GST-Q11

   Plasmid

   #185790

   Purpose

   Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   GST-Q11
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-BCA-Pep-H

   Plasmid

   #185782

   Purpose

   Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   BCA-Pep-H
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a His6Flag2AUF1(p42)

   Plasmid

   #135941

   Purpose

   Expresses AUF1p42 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Use

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-SpyTag002 DA-MBP

   Plasmid

   #102832

   Purpose

   Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002 DA-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Reactive aspartic acid has been mutated to an ala…

   Promoter

   T7

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-ClbN-NHis

   Plasmid

   #51497

   Purpose

   NRPS module containing CATE domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbN
   

   Use

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa30-1094 of NP_754347.1

   Promoter

   T7

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-PTBP1-RRM12CCtoSS

   Plasmid

   #89155

   Purpose

   Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
   

   Use

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Hook3 aa 1-239

   Plasmid

   #74609

   Purpose

   Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a His6Flag2AUF1(p40)

   Plasmid

   #135940

   Purpose

   Expresses AUF1p40 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Use

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 6his R.3-AR GUSpET28a+/pLysS

   Plasmid

   #61157

   Purpose

   N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

   Depositor

   Andrew Ellington, Ichiro Matsumura

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   GUSAR
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

   Promoter

   T7

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Hook3 aa 1-160

    Plasmid

    #74607

    Purpose

    Expresses Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28A-p53 (1-73) (P27A)

    Plasmid

    #62068

    Purpose

    Expression of human p53 (1-73) (P27A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P27A

    Promoter

    T7

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-HaloTag-CNA35

    Plasmid

    #215457

    Purpose

    Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    CNA35
    

    Use

    Tags

    HaloTag

    Expression

    Bacterial

    Mutation

    Includes stop codon

    Promoter

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET28a-RANGAP1-CTD

    Plasmid

    #228947

    Purpose

    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized

    Depositor

    Marcin Suskiewicz

    Insert

    RANGAP1-CTD (RANGAP1 Human)
    

    Use

    Tags

    His tag and thrombine cleavage site : MGSSHHHHHHS…

    Expression

    Bacterial

    Mutation

    contains aa 418-587 only

    Promoter

    T7-lac

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a:His-MBP-PKD

    Plasmid

    #237521

    Purpose

    Recombinant protein expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4_pseudokinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pseudokinase region of RWT4

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a:His-MBP-RWT4Ca

    Plasmid

    #237518

    Purpose

    Recombinant protein expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a:His-MBP-RWT4Ca_noKDup

    Plasmid

    #237535

    Purpose

    Recombinant protein expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca_noKDup
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEt28a-SilkTag-CBM-H6

    Plasmid

    #230903

    Purpose

    Bacterial expression of SilkTag

    Depositor

    Sesilja Aranko

    Article

    Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

    Insert

    SilkTag-CBM-H6
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEt28a-H6-SUMO-SilkCatcher

    Plasmid

    #230902

    Purpose

    Bacterial expression of SilkCatcher

    Depositor

    Sesilja Aranko

    Article

    Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

    Insert

    H6-SUMO-SilkCatcher
    

    Use

    Tags

    HisTag, thrombin, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-ImpBeta1-mEosEM

    Plasmid

    #218933

    Purpose

    Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

    Depositor

    Siegfried Musser

    Article

    Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

    Insert

    Importin Beta (KPNB1 Human)
    

    Use

    Tags

    6xHis and mEos4b (EosEM)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Pchi-HMG

    Plasmid

    #231540

    Purpose

    Expresses the HMG of Pchi Sox with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Sox HMG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-Salhel-HMG

    Plasmid

    #231539

    Purpose

    Expresses the HMG of Salhel Sox-I with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Sox-I HMG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-Myflu-POU

    Plasmid

    #231542

    Purpose

    Expresses the DNA binding domain of Myflu POU with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    POU DBD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-Salhel-POU

    Plasmid

    #231541

    Purpose

    Expresses the DNA binding domain of Salhel POU with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    POU DBD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-OuIAD(E502Q)

    Plasmid

    #226973

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(E502Q)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    E502Q

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-CsHPAD_beta-gamma

    Plasmid

    #226981

    Purpose

    Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    CsHPAD_beta-gamma
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-OuIAD(W392A)

    Plasmid

    #226980

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(W392A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    W392A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-OuIAD(W392F)

    Plasmid

    #226979

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(W392F)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    W392F

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-OuIAD(F401A)

    Plasmid

    #226978

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(F401A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    F401A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-OuIAD(L616E)

    Plasmid

    #226977

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(L616E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    L616E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-OuIAD(R226K)

    Plasmid

    #226976

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226K)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226K

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-OuIAD(R226M)

    Plasmid

    #226975

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226M)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226M

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-OuIAD(R226E)

    Plasmid

    #226974

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-OuIAD(H514E)

    Plasmid

    #226972

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-OuIAD(H514A)

    Plasmid

    #226971

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-OuIAD(C500S)

    Plasmid

    #226970

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(C500S)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    C500S

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-OuIAD(G853A)

    Plasmid

    #226969

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(G853A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    G853A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a(+)-NEDD4L_W4-HECT

    Plasmid

    #187710

    Purpose

    Express his-tagged NEDD4L W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 543-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-542

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

    Plasmid

    #187711

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 with mutations (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491; mutations C702A, C776…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-SpyCatcher -AVI

    Plasmid

    #223635

    Purpose

    Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    AVI tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-FKBP-empty

    Plasmid

    #221332

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    6xHis-3xFKBP empty scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge