We narrowed to 409 results for: AAVS1

1. pODN-AAVS1-mNG-CAAX

   Plasmid

   #183870

   Purpose

   Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-EF1a-mTagRFP-T-CAAX

   Plasmid

   #167973

   Purpose

   AAVS1 targeting repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFP-T-CAAX

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBL437 AAVS1 EQR sgRNA

   Plasmid

   #126447

   Purpose

   To cut the human AAVS1 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human AAVS1 safe-harbor locus with NGAG PAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-Neo-TNNT2-Puro

   Plasmid

   #214016

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-Neo-TNNT2-Zeo

   Plasmid

   #214017

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Puro Xlone SOX17

   Plasmid

   #179838

   Purpose

   Doxycycline-inducible expression of human SOX17

   Depositor

   Xiaoping Bao

   Article

   Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

   Insert

   SOX17 (SOX17 Human)
   

   Use

   AAV, CRISPR, Synthetic Biology, and TALEN ; Donor…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK233 (AAVS1 CMV-AtAFB2)

   Plasmid

   #140537

   Purpose

   AAVS1 CMV-AtAFB2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CMV-AtAFB2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-TRE3G-NODAL-matMYC

   Plasmid

   #115639

   Purpose

   For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL (NODAL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1 Insulator ObLiGaRe/EPB71

    Plasmid

    #90018

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by insulator and inverted ZFN binding sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-SA-CAG-Pa01-BFP

    Plasmid

    #234142

    Purpose

    Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Insert

    Pa01
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1 EGFP-p62 HRD

    Plasmid

    #207550

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-P62
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-Blast-PECAM1-Neo

    Plasmid

    #221149

    Purpose

    Expression of platelet and endothelial cell adhesion molecule 1 (CD31)

    Depositor

    Paul Burridge

    Article

    Pinheiro et al Sci Rep. 2024 Mar 26;14(1):7123. doi: 10

    Insert

    Platelet And Endothelial Cell Adhesion Molecule 1 (PECAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PECAM1

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. SAM-DNMT3A-inactive+sgAAVS1_145

    Plasmid

    #213170

    Purpose

    Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgAAVS1_145
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa and U6

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SAM-DNMT3A-inactive+sgAAVS1_118

    Plasmid

    #213171

    Purpose

    Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

    Depositor

    Sefi Rosenbluh

    Article

    Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

    Insert

    sgAAVS1_118 (AAVS1 Human)
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa and U6

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-TRE-TurboID-HA-rtTA

    Plasmid

    #226995

    Purpose

    Backbone plasmid to express TurboID fusion proteins in mammalian cells

    Depositor

    Bruno Dallagiovanna

    Article

    Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-AAVS1-PGK-Zeo

    Plasmid

    #227271

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

    Plasmid

    #217665

    Purpose

    Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    OsTir1 F74G-SNAP-Blast-AAVS1
    

    Use

    Tags

    SNAP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-Puro XLone-CCND2

    Plasmid

    #179845

    Purpose

    donor plasmid for inducible expression of CCND2 in human cells

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    CRISPR, Luciferase, and TALEN ; Donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-Blasticidin XLone-eGFP

    Plasmid

    #159754

    Purpose

    AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    all-in-one tet-on system
    

    Use

    CRISPR and TALEN; Donor plasmid for targeted knoc…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS inducible promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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