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Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-FLAG
Plasmid#67619PurposeA pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…TagsC-terminal FlagExpressionMammalianMutationPromoterCMV and SP6Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgA2/λ
Plasmid#50371PurposeExpresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG4/λ
Plasmid#50369PurposeExpresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-3HA
Plasmid#67616PurposeA pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…Tagsthree inframe 3' HA tagsExpressionMammalianMutationPromoterCMV and SP6Available sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgA1/λ
Plasmid#50370PurposeExpresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG2/λ
Plasmid#50367PurposeExpresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG3/λ
Plasmid#50368PurposeExpresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
UseTagsExpressionMammalianMutationPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ubc-megf10-GFP
Plasmid#40207DepositorInsertMegf10 (Megf10 Mouse)
UseTagsGFP fusionExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
R777-E253 Hs.RASSF10
Plasmid#70537PurposeGateway ORF clone of human RASSF10 [NM_001080521.2] with stop codon (for native or N-terminal fusions)DepositorInsertRASSF10 (RASSF10 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF10-2xCSTREP
Plasmid#136174PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF10DepositorInsertORF10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF10
Plasmid#169398PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFPDepositorInsertSARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
WEE1 F10.4 gRNA
Plasmid#90941Purpose3rd generation lentiviral gRNA plasmid targeting human WEE1DepositorInsertWEE1 (Guide Designation F10.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-RNF103-CHMP3
Plasmid#140584PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorInsertgRNA RNF103-CHMP3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PF10_0166-bio-His
Plasmid#50801PurposeExpresses enzymatically monobiotinylated full length PF10_0166DepositorInsertPF10_0166
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-F104C
Plasmid#220296Purposemammalian expression of Bcl-2-F104C tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationF104CPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DNAAF10:1-357
Plasmid#211138PurposeMammalian expression of full-length human DNAAF10. N-terminal HiBiT tag.DepositorInsertDNAAF10:M1-I357 (WDR92 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DNAAF10:1-357
Plasmid#211179PurposeMammalian expression of full-length human DNAAF10. C-terminal HiBiT tag.DepositorInsertDNAAF10:M1-I357 (WDR92 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC166-P2F10
Plasmid#203007PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC166DepositorInsertEGFP/BC166
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC262-P3F10
Plasmid#203103PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC262DepositorInsertEGFP/BC262
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only