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Plasmid#177889PurposeBaculo expression of GST tag fused to human CD45 ICDDepositorInsertCD45 (aa 598-1304 only) (PTPRC Human)
UseTagsTEV-GST-PreScission cut siteExpressionInsectMutationL652PPromoterPolyhedrinAvailable sinceAug. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK332
Plasmid#59573PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5DepositorInsertSGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARtcr-IRESpuro
Plasmid#89346Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassetteDepositorInsertTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterSFFVAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(WT)
Plasmid#27134DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-MKL1/2 shRNA
Plasmid#27161PurposeLentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2.DepositorInsertshRNA MKL1+2
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S-pFLAG-CMV1
Plasmid#31384DepositorInserthPDI1 (P4HB Human)
UseTagsFLAG M1ExpressionMammalianMutationC400SPromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-29a-5p
Plasmid#103392PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-5p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK105
Plasmid#59562PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PESTDepositorInsertSGP(14) 2xK-turn EYFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MyD116.delC.pBABEpu
Plasmid#21835DepositorInsertGADD34 (Ppp1r15a Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
TLS 1: hTLS.pBSKS(+)
Plasmid#21827DepositorInsertTLS (FUS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-5p
Plasmid#103723PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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DNA-CARzeta-IRESpuro
Plasmid#89345Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassetteDepositorInsertDNA-CARzeta
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-17b_Unc104-KIF5B-HALO
Plasmid#169996PurposeUltra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labelingDepositorInsertKinesin-like protein Unc-104 fused with KIF5B
UseTagsHALO-tag, 6xHISExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 Ets mut
Plasmid#27157PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationR62E/R65E/Y66DPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
TLS 5:hTLS-CHOP.pCDNA1
Plasmid#21831DepositorInsertTLS-CHOP (FUS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-P2A-strepII-PPPCB
Plasmid#67923PurposeExpresses mCherry and strepII-tag-PP2aBeta in mammalian cellsDepositorInsertmCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -